Potassium bicarbonate and D-ribose effects on A72 canine and HTB-126 human cancer cell line proliferation in vitro

Cancer Cell International

Maurizio Dondi²  Marianna Castaldo¹  Simona Bussolati³  Luca Bruni⁴  Simonetta Croci¹ 

[1] INBB (Istituto Nazionale Biostrutture e Biosistemi) Via delle medaglie d'Oro 305, Roma, Italy;Dipartimento di Salute Animale, Sezione di Clinica Medica Veterinaria, University of Parma, Via del taglio 10, Parma, Italy;Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti-Sezione di Fisiologia Veterinaria, University of Parma, Via del taglio 10, Parma, Italy;Valsè Pantellini Foundation, Calle Cervantes, 16/4 izda, Oviedo Asturia, Spain

关键词: cell proliferation;    cancer;    MTT assay;    D-ribose;    potassium bicarbonate;    HTB-126 human cancer cell line;    A72 canine cancer cell line;   

Others  :  795436
DOI  :  10.1186/1475-2867-11-30

 received in 2011-02-24, accepted in 2011-08-22,  发布年份 2011

【 摘 要 】

Background

The synergic action of KHCO₃ and D-ribose is tested on A72 and HTB-126 cell lines proliferation using K:D-Rib solution. Altered Na⁺/K⁺ ATPase expression and activity were shown in patients with cancer. Studies in human epithelial-derived malignancies indicate that K⁺ depletion also occurs, contributing to the increased intracellular Na⁺/K⁺ ratio [1]. D-ribose transformed to piruvate, enters into the Krebs's cycle and has a key role on energetic metabolism. The up-regulation of glycolysis in tumor cells is already well known and it is the rationale of F¹⁸-FDG PET diagnostic technique. D-ribose is synthesized by the non-oxidative transketolase PPP reaction.

Results

Results with different K:D-Rib concentrations show that MTT salt interferes with K:D-Rib solution and therefore this method is not reliable. The UV/VIS measurements show that K:D-Rib solutions reduce MTT salt to formazan in absence of cells. Cell proliferation has then been evaluated analysing the digital photos of the Giemsa stained cells with MCID™ software. At 5 mM K:D-Rib concentration, the cell growth arrests between 48 h and 72 h; in fact the cell number after 48 h is around the same with respect to the control after 72 h. In case of HTB-126 human cancer cells, the growth rate was valuated counting the splitting times during 48 days: control cells were split sixteen times while 5 mM treated cells eleven times. Most relevant, the clonogenic assay shows that nine colonies are formed in the control cells while only one is formed in the 5 mM and none in 10 mM treated cells.

Conclusions

The K:D-Rib solution has an antioxidant behaviour also at low concentrations. Incubation with 5 mM K:D-Rib solution on A72 cells shows a cytostatic effect at 5 mM, but it needs more than 24 h of incubation time to evidence this effect on cell proliferation. At the same concentration on human HTB-126 cells, K:D-Rib solution shows a clear replication slowing but the cytostatic effect at 10 mM K:D-Rib solution only. Results on A72 cells indicate the K⁺ uptake could be determinant either to arrest or to slow down cell growth.

【 授权许可】

   
2011 Croci et al; licensee BioMed Central Ltd.

【 图 表 】

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