Long non-coding RNA expression profile in human gastric cancer and its clinical significances

Journal of Translational Medicine

Junming Guo³  Yang Xi³  Bingxiu Xiao³  Tian Xia³  Sijie Zhang²  Zhong Liu¹  Xiaoyun Ding²  Guoliang Ye¹  Weiliang Sun⁴  Haojun Song³ 

[1] The Affiliated Hospital, Ningbo University School of Medicine, Ningbo 315010, China;Ningbo No. 1 Hospital and the Affiliated Hospital, Ningbo University School of Medicine, Ningbo 315010, China;Department of Biochemistry and Molecular Biology, and Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, China;Ningbo Yinzhou People’s Hospital and the Affiliated Hospital, Ningbo University School of Medicine, Ningbo 315040, China

关键词: uc001lsz;    H19;    Expression profile;    Long non-coding RNA;    Gastric cancer;   

Others  :  826373
DOI  :  10.1186/1479-5876-11-225

 received in 2013-08-24, accepted in 2013-09-18,  发布年份 2013

【 摘 要 】

Background

Long non-coding RNAs (lncRNAs) are prevalently transcribed in the genome yet their potential roles in human cancers are not well understood. The aim of the present study was to determine the lncRNA expression profile in gastric cancer and its potential clinical value.

Methods

The global lncRNA expression profile in gastric cancer was measured by lncRNA microarray. Levels of two representative lncRNAs, H19 and uc001lsz, were confirmed by real-time reverse transcriptase-polymerase chain reaction. The relationship between their levels and clinicopathological factors of patients with gastric cancer was explored. A receiver operating characteristic (ROC) curve was constructed for differentiating gastric cancer from benign gastric diseases.

Results

Total of 135 lncRNAs, which differential expression levels between tumor and non-tumorous tissues were more than twofold, were found (GEO No. GSE47850). The most down-regulated lncRNAs in gastric cancer tissues were FER1L4, uc001lsz, BG491697, AF131784, uc009ycs, BG981369, AF147447, HMlincRNA1600, and AK054588; while the most up-regulated ones were H19, HMlincRNA717, BM709340, BQ213083, AK054978, and DB077273. H19 was found highly expressed in stomach and liver cancer cell lines, while lowly expressed in lung cancer and prostate cancer cell lines. Uc001lsz was lowly expressed in gastric, lung and liver cancer cell lines, while highly expressed in prostate cancer. The areas under ROC curves were up to 0.613, 0.751, and 0.761 for H19, uc001lsz, and the combination, respectively.

Conclusions

The lncRNA expression profile in gastric cancer suggests the potential roles of lncRNAs in gastric cancer occurrence and development. The overexpression of H19 in gastric cancer suggests that H19 may be participated in gastric cancer. The reduced expression of uc001lsz in gastric cancer cell lines and tissues, its associations with TNM stage, and its dysregulation in early cancer and precancerous lesions suggest that uc001lsz may be a potential marker for the diagnosis of early gastric cancer.

【 授权许可】

   
2013 Song et al.; licensee BioMed Central Ltd.

【 图 表 】

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