Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil

Journal of Environmental Health Science Engineering

Sudheer Khan²  Preethy Chandran²  NS Vasanthi¹  Srilakshman Kumar¹  Jayaprakash Ramya¹  Nandhini Palanisamy¹ 

[1] Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India;CeNTAB, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu, India

关键词: GC-MS analysis;    Biodegradation;    Parameter optimization;    Diesel oil;    Acinetobacter baumannii;   

Others  :  1175276
DOI  :  10.1186/s40201-014-0142-2

 received in 2013-10-23, accepted in 2014-11-22,  发布年份 2014

【 摘 要 】

Petroleum based products are the major source of energy for industries and daily life. Leaks and accidental spills occur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleum products. In the present study we isolated the bacteria from diesel contaminated soil and screened them for diesel biodegradation capacity. One monoculture isolate identified by 16S rRNA gene sequence analysis to be Acinetobacter baumannii was further studied for diesel oil biodegradation. The effects of various culture parameters (pH, temperature, NaCl concentrations, initial hydrocarbon concentration, initial inoculum size, role of chemical surfactant, and role of carbon and nitrogen sources) on biodegradation of diesel oil were evaluated. Optimal diesel oil biodegradation by A. baumanii occurred at initial pH 7, 35°C and initial hydrocarbon concentration at 4%. The biodegradation products under optimal cultural conditions were analyzed by GC-MS. The present study suggests that A. baumannii can be used for effective degradation of diesel oil from industrial effluents contaminated with diesel oil.

【 授权许可】

   
2014 Palanisamy et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Kingston PF: Long-term environmental impact of oil spills. Spill Sci Technol Bull 2007, 7:53-61.
• [2]Gallego JLR, Loredo J, Llamas JF, Vázquez F, Sánchez J: Bioremediation of diesel-contaminated soils: evaluation of potential in situ techniques by study of bacterial degradation. Biodegradation 2001, 12:325-335.
• [3]Pala DM, De Carvalho DD, Pinto JC, Sant Anna Jr GL: Suitable model to describe bioremediation of a petroleum contaminated soil. Int Biodeterior Biodegrad 2006, 58:254-260.
• [4]Makkar RS, Rockne KJ: Comparison of synthetic surfactants and biosurfactants in enhancing biodegradation of polycyclic aromatic hydrocarbons. Environ Toxicol Chem 2003, 22:2280-2292.
• [5]Mukherji S, Vijay A: Critical issues in bioremediation of oil and tar contaminated sites, In: Proceedings of the International Conference on Advances in Civil Engineering.Civil Eng Dept 2002, 507-516
• [6]Holt JG, Krieg NR, Sneath PHA: Bergey’s Manual of Determinative Bacteriology. 9th edition. Williamsand Wilkins, Baltimore; 1994.
• [7]Buchanan RE, Gibbons NE: Bergey’s Manual of Determinative Bacteriology. Science Press, Beijing; 1984.
• [8]Zhenle T, Lihua Z, Yinghui W, Heqing T: Evalution of biodegradation of petroleum hydrocarbons pollutions by gas chromatography and spectrophotometry. Chin J Analyt Chem 2006, 34:343-346.
• [9]Haritash AK, Kaushik CP: Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs): a review. J Hazard Mater 2009, 169:1-15.
• [10]Whang L-M, Liu P-WG, Ma C-C, Cheng S-S: Application of rhamnolipid and surfactin for enhanced diesel biodegradation-Effects of pH and ammonium addition. J Hazard Mater 2009, 164:1045-1050.
• [11]Xia W, Li J, Xia Y, Song Z, Zhou J: Optimization of diesel oil biodegradation in seawater using statistical experimental methodology. Water Sci Technol 2012, 66:1301-1309.
• [12]Luo Q, Zhang J-G, Shen X-R, Sui X, Fan Z-Q: Characterization of a novel diesel oil-degrading pseudomonas sp. strain F4. Fresenius Environ Bullet 2013, 22:689-697.
• [13]Sathishkumar M, Binupriya AR, Baik S-H, Yun S-E: Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon contaminated areas. Clean-Soil Air Water 2008, 36:92-96.
• [14]Mnif I, Sahnoun R, Ellouze-Chaabouni S, Ghribi D: Evaluation of B. subtilis SPB1 biosurfactants’ potency for diesel-contaminated soil washing: optimization of oil desorption using Taguchi design. Environ Sci Pollut Res 2014, 21:851-861.
• [15]Luo Q, Xian-Rong S, Jian-Guo Z, Zheng-Qiu F, Ying H: Isolation, identification and biodegradation ability of diesel oil degrading Pseudomonas sp. strain C7 from bilge water. Afr J Microbiol 2012, 6:1033-1040.
• [16]Greenwood PF, Wibrow S, George SJ, Tibbett M: Sequential hydro- carbon biodegradation in a soil from arid coastal Australia treated with oil under laboratory controlled conditions. Org Geochem 2008, 39:1336-1346.
• [17]Lakshmi MB, Muthukumar K, Velan M: Immobilization of mycoplana sp. mvmb2 isolated from petroleum contaminated soil onto papaya stem (carica papaya l.) and its application on degradation of phenanthrene. Clean-Soil Air Water 2012, 40:870-877.
• [18]Mnif S, Sayadi S, Chamkha M: Biodegradative potential and characterization of a novel aromatic-degrading bacterium isolated from a geothermal oil field under saline and thermophilic conditions. Int Biodeterior Biodegrad 2014, 86:258-264.
• [19]Prathima Devi M, Venkateswar Reddy M, Juwarkar A, Nageswara Sarma P, Venkata Mohan SR: Effect of co-culture and nutrients supplementation on bioremediation of crude petroleum sludg. Clean-Soil Air Water 2011, 39:900-907.
• [20]Ganesh A, Lin B: Diesel degradation and biosurfactant production by Gram- positive isolate. Afr J Biotechnol 2009, 8:5847-5854.
• [21]Zahed MA, Aziz HA, Isa MH, Mohajeri L: Response surface analysis to improve dispersed crude oil biodegradation. Clean-Soil Air Water 2012, 40:262-267.
• [22]Liu CW, Chang WN, Liu H-S: Speedy Hydrocarbon Pollutants Treatment Through the Cell Interaction by a Novel Strain Rhodococcus: Its Fundamental Characteristics and Applications. 238th National Meeting and Exposition of the American Chemical Society, ACS, Washington, DC; United States; 2009.
• [23]Delgado JA, Follett RF: Nitrogen fate and transport in agricultural systems. J Soil Water Conserv 2002, 57:402-407.
• [24]Bautista LF, Sanz R, Molina MC, González N, Sánchez D: Effect of different non-ionic surfactants on the biodegradation of PAHs by diverse bacteria. Int Biodeter Biodegr 2009, 63:913-922.
• [25]Ilori MO, Adebusoye SA, Ojo AC: Isolation and characterization of hydrocarbon degrading and biosurfactant producing yeast strains obtained from lagoon water. World J Microbiol Biotechnol 2008, 24:2539-2545.
• [26]Chandran P, Das N: Degradation of diesel oil by immobilized Candida tropicalis and biofilm formed on gravels. Biodegradation 2011, 22:1181-1189.