Viral and cellular SOS-regulated motor proteins: dsDNA translocation mechanisms with divergent functions

Cell & Bioscience

Tao Weitao¹  Kara Phipps¹  Annie Wolfe¹ 

[1] Biology Department, College of Science and Mathematics, Southwest Baptist University, 1600 University Ave, Bolivar, Missouri 65613, USA

关键词: Phages;    Bacteria;    SOS response;    Recombination;    Replication;    DNA repair;    DNA translocases;    AAA+ proteins;   

Others  :  790442
DOI  :  10.1186/2045-3701-4-31

 received in 2014-02-06, accepted in 2014-06-13,  发布年份 2014

【 摘 要 】

DNA damage attacks on bacterial cells have been known to activate the SOS response, a transcriptional response affecting chromosome replication, DNA recombination and repair, cell division and prophage induction. All these functions require double-stranded (ds) DNA translocation by ASCE hexameric motors. This review seeks to delineate the structural and functional characteristics of the SOS response and the SOS-regulated DNA translocases FtsK and RuvB with the phi29 bacteriophage packaging motor gp16 ATPase as a prototype to study bacterial motors. While gp16 ATPase, cellular FtsK and RuvB are similarly comprised of hexameric rings encircling dsDNA and functioning as ATP-driven DNA translocases, they utilize different mechanisms to accomplish separate functions, suggesting a convergent evolution of these motors. The gp16 ATPase and FtsK use a novel revolution mechanism, generating a power stroke between subunits through an entropy-DNA affinity switch and pushing dsDNA inward without rotation of DNA and the motor, whereas RuvB seems to employ a rotation mechanism that remains to be further characterized. While FtsK and RuvB perform essential tasks during the SOS response, their roles may be far more significant as SOS response is involved in antibiotic-inducible bacterial vesiculation and biofilm formation as well as the perspective of the bacteria-cancer evolutionary interaction.

【 授权许可】

   
2014 Wolfe et al.; licensee BioMed Central Ltd.

【 图 表 】

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