Fructose-rich diet leads to reduced aerobic capacity and to liver injury in rats

Lipids in Health and Disease

Maria Alice Rostom de Mello¹  Fabrício Azevedo Voltarelli²  Rodrigo Augusto Dalia¹  Ana Carolina Ghezzi¹  Lucieli Teresa Cambri¹  José Diego Botezelli¹ 

[1] Department of Physical Education, São Paulo State University - UNESP, Av: 24-A, 1515 Bela Vista., 13506-900, Rio Claro, São Paulo, Brazil;Department of Physical Education, Federal University of Mato Grosso, Grosso, MT, Brazil

关键词: Rats;    Oxidative stress;    Liver injury;    Fructose;   

Others  :  1160271
DOI  :  10.1186/1476-511X-11-78

 received in 2011-12-28, accepted in 2012-06-19,  发布年份 2012

【 摘 要 】

The main purpose of this research was to investigate the alterations in the aerobic capacity and appearance of metabolic alterations in Wistar rats fed on fructose-rich diet. We separated twenty-eight rats into two groups according to diet: a control group (C) (balanced diet) and a fructose-rich diet group (F). The animals were fed these diets for 60 d (d 120 to 180). We performed insulin, glucose as well as a minimum lactate test, at d 120 and 180. At the end of the experiment, sixteen animals were euthanized, and the following main variables were analysed: aerobic capacity, the serum aspartate aminotransferase (AST) to alanine aminotransferase (ALT) ratio, serum and liver triglyceride concentrations, serum and liver thiobarbituric acid reactive substance (TBARS) concentrations, serum and liver catalase and superoxide dismutase (SOD) activity and haematoxylin-eosin histology (HE) in hepatocytes. The remaining twelve animals were submitted to an analysis of their hepatic lipogenic rate. The animals fed a fructose-rich diet exhibited a reduction in aerobic capacity, glucose tolerance and insulin sensitivity and increased concentrations of triglycerides and TBARS in the liver. Catalase and SOD activities were reduced in the livers of the fructose-fed animals. In addition, the serum AST/ALT ratio was higher than that of the C group, which indicates hepatic damage, and the damage was confirmed by histology. In conclusion, the fructose-rich diet caused significant liver damage and a reduction in insulin sensitivity in the animals, which could lead to deleterious metabolic effects.

【 授权许可】

   
2012 Botezelli et al.; licensee BioMed Central Ltd.

【 图 表 】

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