Antimicrobial resistance in commensal faecal Escherichia coli of hospitalised horses

Irish Veterinary Journal

Vivienne Duggan²  Lisa Katz²  Séamus Fanning³  Nola Leonard¹  Jill Bryan² 

[1]Veterinary Pathobiology and Infectious Diseases, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland

[2]Veterinary Clinical Studies, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland

[3]Centre for Food Safety, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland

关键词: Escherichia coli;    hospitalisation;    resistance;    antimicrobial;    Horse;   

Others  :  1149627
DOI  :  10.1186/2046-0481-63-6-373

【 摘 要 】

The objective of this study was to examine the impact of hospitalisation and antimicrobial drug administration on the prevalence of resistance in commensal faecal E. coli of horses. Faecal samples were collected from ten hospitalised horses treated with antimicrobials, ten hospitalised horses not treated with antimicrobials and nine non-hospitalised horses over a consecutive five day period and susceptibility testing was performed on isolated E. coli. Results revealed that hospitalisation alone was associated with increased prevalence of antimicrobial resistance and multidrug resistance in commensal E. coli of horses. Due to the risk of transfer of resistance between commensal and pathogenic bacteria, veterinarians need to be aware of possible resistance in commensal bacteria when treating hospitalised horses.

   

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【 参考文献 】

• [1]Anzai T, Kamada M, Ike K, et al.: Drug susceptibility of Escherichia coli isolated from foals with diarrhea and mares with metritis. Bulletin of Equine Research Institute 1987, 24:42-50.
• [2]Blake DP, Hillman K, Fenlon DR, et al.: Transfer of antibiotic resistance between commensal and pathogenic members of the Enterobacteriaceae under ileal conditions. J Appl Microbiol 2003, 95:428-436.
• [3]Boerlin P, Eugster S, Gaschen F, et al.: Transmission of opportunistic pathogens in a veterinary teaching hospital. Vet Microbiol 2001, 82:347-359.
• [4]Bucknell DG, Gasser RB, Irving A, et al.: Antimicrobial resistance in Salmonella and Escherichia coli isolated from horses. Aust Vet J 1997, 75:355-356.
• [5]CLSI: Performance standards for antimicrobial disk diffusion susceptibility tests; Approved Standard. Clinical and Laboratory Standards Institute, Pennsylvania 2005.
• [6]Collignon P, Aarestrup FM: Extended-spectrum β-lactamases, food, and cephalosporin use in food animals. Clin Infect Dis 2007, 44:1391-1392.
• [7]Dargatz DA, Traub-Dargatz JL, Sangster NC: Antimicrobic and anthelmintic resistance. Vet Clin North Am Equine Pract 2000, 16:515-536.
• [8]Dunowska M, Morley PS, Traub-Dargatz JL, et al.: Impact of hospitalization and antimicrobial drug administration on antimicrobial susceptibility patterns of commensal Escherichia coli isolated from the feces of horses. J Am Vet Med Assoc 2006, 228:1909-1917.
• [9]Guérin-Faublée V, Carret G, Houffschmitt P: In vitro activity of 10 antimicrobial agents against bacteria isolated from cows with clinical mastitis. Vet Rec 2003, 152:466-471.
• [10]Gustafsson I, Sjölund M, Torell E, et al.: Bacteria with increased mutation frequency and antibiotic resistance are enriched in the commensal flora of patients with high antibiotic usage. J Antimicrob Chemother 2003, 52:645-650.
• [11]Levy SB, Marshall B: Antibacterial resistance worldwide: causes, challenges and responses. Nat Med Supplement 2004, 10:S122-129.
• [12]Li XZ, Mehrotra M, Ghimire S, et al.: β-Lactam resistance and β-lactamases in bacteria of animal origin. Vet Microbiol 2007, 121:197-214.
• [13]McEwen SA, Fedorka-Cray PJ: Antimicrobial use and resistance in animals. Clin Infect Dis 2002, 34(Suppl 3):S93-S106.
• [14]Morley PS, Apley MD, Besser TE, et al.: Antimicrobial drug use in veterinary medicine. J Vet Int Med 2005, 19:617-629.
• [15]Mulvey MR, Simor AE: Antimicrobial resistance in hospitals: How concerned should we be? CMAJ 2009, 180:408-415.
• [16]Paterson DL: Resistance in gram-negative bacteria: Enterobacteriaceae. Am J Infect Control 2006, 34:S20-28.
• [17]Prescott JF: Antimicrobial drug resistance and its epidemiology. In Antimicrobial Therapy in Veterinary Medicine. Edited by Prescott JF, Baggot JD, Walker RD. Ames, Iowa State University Press; 2000:35-36.
• [18]Seguin JC, Walker RD, Caron JP, et al.: Methicillin-resistant Staphylococcus aureus outbreak in a veterinary teaching hospital: potential human-to-animal transmission. J Clin Microbiol 1999, 37:1459-1463.
• [19]Southwood LL: Principles of antimicrobial therapy: what should we be using? Vet Clin North Am Equine Pract 2006, 22:279-296.
• [20]Tenover FC: Mechanisms of antimicrobial resistance in bacteria. Am J Med 2006, 119:S3-S10.
• [21]van de Sande-Bruinsma N, Grundmann H, Verloo D, et al.: European antimicrobial resistance surveillance system and European surveillance of antimicrobial consumption project groups. Antimicrobial drug use and resistance in Europe. Emerg Infect Dis 2008, 14:1722-1730.
• [22]van den Bogaard AE, Stobberingh EE: Antibiotic usage in animals: impact on bacterial resistance and public health. Drugs 1999, 58:589-607.
• [23]Vo ATT, van Duijkeren E, Fluit AC, et al.: Characteristics of extended-spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates from horses. Vet Microbiol 2007, 124:248-255.
• [24]Ward MP, Brady TH, Couëtil LL, et al.: Investigation and control of an outbreak of salmonellosis caused by multidrug-resistant Salmonella Typhimurium in a population of hospitalized horses. Vet Microbiol 2005, 107:233-240.
• [25]Weese JS, Lefebvre SL: Risk factors for methicillin-resistant Staphylococcus aureus colonization in horses admitted to a veterinary teaching hospital. Can Vet J 2007, 48:921-926.