Flavonoids furom Coreopsis tinctoria adjust lipid metabolism in hyperlipidemia animals by down-regulating adipose differentiation-related protein

Lipids in Health and Disease

Muhuyati Wulasihan¹  Xinhua Chen²  Jiangyun Liu⁴  Jie Xue⁴  Xinmei Chen³  Yali Li¹ 

[1] The Department of Internal Medicine, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang 830000, China;The Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China;The Department of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan Shandong 250014, China;College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China

关键词: Adipose differentiation-related protein (ADRP);    Fenofibrate;    Hyperlipemia;    Flavonoid;    Coreopsis tinctoria;   

Others  :  1145515
DOI  :  10.1186/1476-511X-13-193

 received in 2014-07-25, accepted in 2014-11-30,  发布年份 2014

【 摘 要 】

Background

To identify the chemical structure of Coreopsis tinctoria extracts and their effect and mechanism on reducing blood lipid in hyperlipemia mice.

Methods

The flavonoids were extracted from Coreopsis tinctoria. The chemical structure was identified by HPLC. 59 mice were divided randomly into 5 groups. (group 1: normal diet control; group 2: hyperlipemia model; group 3: hyperlipemia mice treated with Coreopsis tinctoria, low dose 100 mg/kg; group 4: hyperlipemia mice treated with Coreopsis tinctoria high dose group 200 mg/kg; group 5 hyperlipemia mice treated with Fenofibrate. After 2 week of hyperlipid diet, the treatment of Coreopsis tinctoria and Fenofibrate were given for another 6 weeks with continuous hyperlipid diet. The TC, TG, HDL, histology, adipose differentiation-related protein (ADRP) expression in different groups were compared.

Results

Compared with normal diet group, TC, TG in hyperlipemia model group increased ( P < 0. 01). After treatment with Coreopsis tinctoria low dose group, high dose group, TC of the hyperlipemia mice decreased (P < 0. 05) without increasing AST, ALT and ALP. Fenofibrate can also decrease TC and TG but increase AST, ALT and ALP. Expression of hepatic ADRP increased in hyperlipemia mice. Coreopsis tinctoria high dose group 200 mg/kg can inhibit ADRP as Fenofibrate does.

Conclusion

The flavonoids from Coreopsis tinctoria extracts can reduce blood lipid without liver function damage, showing better anti- hyperlipemia effect than Fenofibrate by down-regulating ADRP.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

【 参考文献 】

• [1]Del Ben M, Polimeni L, Baratta F, Pastori D, Loffredo L, Angelico F: Modern approach to the clinical management of non-alcoholic fatty liver disease. World J Gastroenterol 2014, 20(26):8341-8350.
• [2]Scarisbrick JJ, Morris S, Azurdia R, Illidge T, Parry E, Graham-Brown R, Cowan R, Gallop-Evans E, Wachsmuth R, Eagle M, Wierzbicki AS, Soran H, Whittaker S, Wain EM: U.K. consensus statement on safe clinical prescribing of bexarotene for patients with cutaneous T-cell lymphoma. Br J Dermatol 2013, 168(1):192-200.
• [3]Kma L: Plant extracts and plant-derived compounds: promising players in a countermeasure strategy against radiological exposure. Asian Pac J Cancer Prev 2014, 15(6):2405-2425.
• [4]Sun YH, Zhao J, Jin HT, Cao Y, Ming T, Zhang LL, Hu MY, Hamlati H, Pang SB, Ma XP: Vasorelaxant effects of the extracts and some flavonoid from the buds of Coreopsis tinctoria. Pharm Biol 2013, 51(9):1158-1164.
• [5]Dias T, Bronze MR, Houghton PJ, Mota-Filipe H, Paulo A: The flavonoid-rich fraction of Coreopsis tinctoria promotes glucose tolerance regain through pancreatic function recovery in streptozotocin-induced glucose-intolerant rats. J Ethnopharmacol 2010, 132(2):483-490.
• [6]Dias T, Liu B, Jones P, Houghton PJ, Mota-Filipe H, Paulo A: Cytoprotective effect of Coreopsis tinctoria extracts and flavonoids on tBHP and cytokine-induced cell injury in pancreatic MIN6 cells. J Ethnopharmacol 2012, 139(2):485-492. doi:10.1016/j.jep.2011.11.038. Epub 2011 Nov 28
• [7]Salvamani S, Gunasekaran B, Shaharuddin NA, Ahmad SA, Shukor MY: Antiartherosclerotic effects of plant flavonoids. Biomed Res Int 2014, 2014:480258.
• [8]Sharma K, Kumar K, Mishra N: Nanoparticulate carrier system: a novel treatment approach forhyperlipidemia. Drug Deliv 2014, 6:1-16.
• [9]Berlanga A, Guiu-Jurado E, Porras JA, Auguet T: Molecular pathways in non-alcoholic fatty liver disease. Clin Exp Gastroenterol 2014, 7:221-239.
• [10]Rull A, Geeraert B, Aragonès G, Beltrán-Debón R, Rodríguez-Gallego E, García-Heredia A, Pedro-Botet J, Joven J, Holvoet P, Camps J: Rosiglitazone and fenofibrate exacerbate liver steatosis in a mouse model of obesity and hyperlipidemia. A transcriptomic and metabolomic study. J Proteome Res 2014, 13(3):1731-1743.
• [11]Tarantino G, Finelli C, Scopacasa F, Pasanisi F, Contaldo F, Capone D, Savastano S: Circulating levels of sirtuin 4, a potential marker of oxidative metabolism, related to coronary artery disease in obese patients suffering from NAFLD, with normal or slightly increased liver enzymes. Oxid Med Cell Longev 2014, 2014:920676.
• [12]Grasselli E, Voci A, Pesce C, Canesi L, Fugassa E, Gallo G, Vergani L: PAT protein mRNA expression in primary rat hepatocytes: effects of exposure to fatty acids. Int J Mol Med 2010, 25(4):505-512.