Aluminum alters NMDA receptor 1A and 2A/B expression on neonatal hippocampal neurons in rats

Journal of Biomedical Science

Yih-Jing Lee³  Guoo-Shyng Wang Hsu²  Chia-Yi Yuan¹ 

[1] Graduate Institute of Nutrition and Food Sciences, Fu-Jen Catholic University, 510 Chung-Cheng Road, Hsinchuang, New Taipei City 24205, Taiwan;Department of Nutritional Science, Fu-Jen Catholic University, 510 Chung-Cheng Road, Hsinchuang, New Taipei City 24205, Taiwan;School of Medicine, Fu-Jen Catholic University, 510 Chung-Cheng Road, Hsinchuang, New Taipei City 24205, Taiwan

关键词: immunocytochemistry;    N-methyl-D-aspartate receptors;    primary hippocampal neuron;    neonates;    aluminum;   

Others  :  1146845
DOI  :  10.1186/1423-0127-18-81

 received in 2011-07-19, accepted in 2011-11-08,  发布年份 2011

【 摘 要 】

Background

High aluminum (Al) content in certain infant formula raises the concern of possible Al toxicity on brain development of neonates during their vulnerable period of growing. Results of in vivo study showed that Al content of brain tissues reached to 74 μM when oral intake up to 1110 μM, 10 times of that in the hi-Al infant formula.

Methods

Utilizing a cultured neuron cells in vitro model, we have assessed Al influence on neuronal specific gene expression alteration by immunoblot and immunohistochemistry and neural proliferation rate changes by MTT assay.

Results

Microscopic images showed that the neurite outgrowth of hippocampal neurons increased along with the Al dosages (37, 74 μM Al (AlCl₃)). MTT results also indicated that Al increased neural cell viability. On the other hand, the immunocytochemistry staining suggested that the protein expressions of NMDAR 1A and NMDAR 2A/B decreased with the Al dosages (p < 0.05).

Conclusion

Treated hippocampal neurons with 37 and 74 μM of Al for 14 days increased neural cell viability, but hampered NMDAR 1A and NMDAR 2A/B expressions. It was suggested that Al exposure might alter the development of hippocampal neurons in neonatal rats.

【 授权许可】

   
2011 Yuan et al; licensee BioMed Central Ltd.

【 图 表 】

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