Presumable role of outer membrane proteins of Salmonella containing sialylated lipopolysaccharides serovar Ngozi, sv. Isaszeg and subspecies arizonae in determining susceptibility to human serum

Gut Pathogens

Gabriela Bugla-Płoskońska⁴  Jacek Rybka³  Elżbieta Klausa¹  Agata Dorotkiewicz-Jach²  Katarzyna Guz-Regner⁴  Urszula Godlewska⁴  Bożena Futoma-Kołoch⁴ 

[1] Regional Centre of Transfusion Medicine and Blood Bank, Czerwonego Krzyża 5, Wrocław, 50-345, Poland;Department of Pathogens’ Biology and Immunology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63-77, Wrocław, 51-148, Poland;Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, Wrocław, 53-114, Poland;Department of Microbiology, Institute of Genetics and Microbiology, University of Wrocław, Przybyszewskiego 63-77, Wrocław, 51-148, Poland

关键词: Outer membrane protein;    C3;    Complement;    Sialic acid;    Lipopolysaccharide;    Salmonella O48;   

Others  :  1219652
DOI  :  10.1186/s13099-015-0066-0

 received in 2015-05-18, accepted in 2015-07-07,  发布年份 2015

【 摘 要 】

Background

The O48 group comprises Salmonella bacteria containing sialic acid in the lipopolysaccharide (LPS). Bacteria with sialylated surface structures are described as pathogens that avoid immunological response of the host by making similar their surface antigens to the host’s tissues (molecular mimicry). It is known that the smooth-type LPS of Salmonella enterica and outer membrane proteins (OMP) PgtE, PagC and Rck mediate serum resistant phenotype by affecting complement system (C). The aim of this study was to investigate C3 component activation by Salmonella O48 LPS and OMP.

Findings

In the present study, we examined C3 component deposition on the three Salmonella O48 strains: S. enterica subspecies enterica serovar Ngozi, S. enterica subsp. enterica sv. Isaszeg, and S. enterica subsp. arizonae containing sialic acid in the O-specific part of LPS. The greatest C3 deposition occurred on Salmonella sv. Isaszeg cells (p < 0.005) as well as on their LPS (low content of sialic acid in LPS) (p < 0.05) after 45 min of incubation in 50% human serum. Weaker C3 deposition ratio on the Salmonella sv. Ngozi (high content of sialic acid in LPS) and Salmonella subsp. arizonae (high content of sialic acid in LPS) cells correlated with the lower C3 activation on their LPS. Immunoblotting revealed that OMP isolated from the tested strains also bound C3 protein fragments.

Conclusions

We suggest that activation of C3 serum protein is dependent on the sialic acid contents in the LPS as well as on the presence of OMP in the range of molecular masses of 35–48 kDa.

【 授权许可】

   
2015 Futoma-Kołoch et al.

【 图 表 】

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