Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity

Genome Biology

Hiroki R Ueda⁶  Takeshi Imai⁴  Kenichiro D Uno¹  Hiroki Danno²  Tetsutaro Hayashi⁵  Itoshi Nikaido³  Yohei Sasagawa³ 

[1] Functional Genomics Unit, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan;Laboratory for Systems biology, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan;Bioinformatics Research Unit, Advanced Center for Computing and Communication, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;JST, PRESTO, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan;Genome Resource and Analysis Unit, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan;Laboratory for Synthetic Biology, Quantitative Biology Center, RIKEN, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan

关键词: Cell biology;    Cellular heterogeneity;    Bioinformatics;    Sequencing;    Transcriptome;    RNA-seq;    Single cell;   

Others  :  866867
DOI  :  10.1186/gb-2013-14-4-r31

 received in 2012-12-21, accepted in 2013-04-17,  发布年份 2013

【 摘 要 】

Development of a highly reproducible and sensitive single-cell RNA sequencing (RNA-seq) method would facilitate the understanding of the biological roles and underlying mechanisms of non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity than existing methods. We show that single-cell Quartz-Seq can quantitatively detect various kinds of non-genetic cellular heterogeneity, and can detect different cell types and different cell-cycle phases of a single cell type. Moreover, this method can comprehensively reveal gene-expression heterogeneity between single cells of the same cell type in the same cell-cycle phase.

【 授权许可】

   
2013 Sasagawa et al.; licensee BioMed Central Ltd.

【 图 表 】

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