Frequency of D222G haemagglutinin mutant of pandemic (H1N1) pdm09 influenza virus in Tunisia between 2009 and 2011

Diagnostic Pathology

Amine Slim²  Inmaculada Casas¹  Maria Teresa Cuevas¹  Juan Ledesma¹  Francisco Pozo¹  Mohamed Ali Ben Hadj Kacem²  Awatef El Moussi² 

[1] National Influenza Centre-Madrid, Influenza and Respiratory Viruses Laboratory, Instituto de Salud Carlos III, Madrid, Spain;National Influenza Centre-Tunis, Unit Virology, Microbiology Laboratory, Charles Nicolle’s Hospital, Tunis, Tunisia

关键词: Severe respiratory infection;    Pandemic;    Influenza A(H1N1)pdm09 virus;    Haemagglutinin;    D222G substitution;   

Others  :  805949
DOI  :  10.1186/1746-1596-8-124

 received in 2012-09-24, accepted in 2013-03-10,  发布年份 2013

【 摘 要 】

Background

The novel pandemic A (H1N1) pdm09 virus was first identified in Mexico in April 2009 and since then it spread worldwide over a short period of time. Although the virus infection is generally associated with mild disease and a relatively low mortality, it is projected that mutations in specific regions of the viral genome, especially within the receptor binding domain of the haemagglutinin (HA) protein could result in more virulent virus stains, leading to a more severe pathogenicity.

Methods

To monitor the genetic polymorphisms at position 222 of Haemagglutinin of influenza A(H1N1)pdm09 viruses from both outpatients with mild influenza and individuals with severe disease requiring hospitalization, during 2009–2010 and 2010–2011 seasons, a sequence-based genotypic assessment of viral populations to understand the prevalence of D222G mutation.

Results

The D222G was identified in clinical specimens from 3 out of 42 cases analyzed in Tunisia with severe outcome (7%). Interestingly, in one fatal case out of four viruses taken from fatal cases studied (25%). Also this mutation was found in one mild case out of 8 mild cases studied (0.1%). D222E substitution was found in virus taken from one patient with severe clinical syndrome (2%) out of 42 severe cases analyzed and E374K substitution was found in two severe cases (4%) out of 42 severe cases studied.

Conclusions

A specific mutation in the viral haemagglutinin (D222G) was found in fatal, severe and mild case. Further virological, clinical and epidemiological investigations are needed to ascertain the role of this and other mutations that may alter the virulence and transmissibility of the pandemic influenza A (H1N1)pdm09.

Virtual Slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1027334947811255 webcite

【 授权许可】

   
2013 El Moussi et al.; licensee BioMed Central Ltd.

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