Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH)

Genetics Selection Evolution

Patrice Humblot¹  Bernard Guérin¹  Edmond-Paul Cribiu¹  Daniel Vaiman¹  François Piumi¹ 

[1] Laboratoire de radiobiologie et d'étude du génome, Institut national de la recherche agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France

关键词: bacterial artificial chromosome;    Y chromosome;    X chromosome;    spermatozoon;    fluorescent in situ hybridization;   

Others  :  1094711
DOI  :  10.1186/1297-9686-33-1-89

 received in 2000-08-14, accepted in 2000-10-24,  发布年份 2001

【 摘 要 】

X and Y specific probes were identified in order to apply the fluorescent in situ hybridization (FISH) technique to bovine spermatozoa. For Y chromosome detection, the BRY4a repetitive probe, covering three quarters of the chromosome, was used. For X chromosome detection, a goat Bacterial Artificial Chromosome (BAC) specific to the X chromosome of bovine and goats and giving a strong FISH signal was used. Each probe labeled roughly 45% of sperm cells. The hybridization method will be useful for evaluating the ratio of X- and Y- bearing spermatozoa in a sperm sample and consequently can be used to evaluate the efficiency of sperm sorting by different techniques such as flow cytometry.

【 授权许可】

   
2001 INRA, EDP Sciences