Cigarette smoke impairs granulosa cell proliferation and oocyte growth after exposure cessation in young Swiss mice: an experimental study

Journal of Ovarian Research

Celly Cristina A Nascimento-Saba¹  Poli Mara Spritzer³  Rachel MM Santos²  Aluana C Santana¹  Aline S Santos¹  Gabrielle PL Gonzalez¹  Rejane P Gaspar-Reis¹  Larissa LO Paixão¹ 

[1] Department of Physiological Sciences, Institute of Biology Roberto Alcântara Gomes, University of the State of Rio de Janeiro, Av. 28 de Setembro, 87 PAPC. Vila Isabel, 20551-030 , Rio de Janeiro, Brazil;Department of Physiology and Pharmacology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Melo, 101. São Domingos, Niterói 24210-130, Niterói, Brazil;Gynecological Endocrinology Unit, Division of Endocrinology, Hospital de Clinicas de Porto Alegre and Department of Physiology, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos 2350, 90035-003, Porto Alegre, Brazil

关键词: Smoke cessation;    Antral follicles;    Morphometric aspects;    Ovary;    Female mice;    Folliculogenesis;    Oocyte;    Granulosa cell;    Cigarette smoking;    Tobacco;   

Others  :  814989
DOI  :  10.1186/1757-2215-5-25

 received in 2012-08-11, accepted in 2012-09-17,  发布年份 2012

【 摘 要 】

Background

Cigarette smoke is associated with decreased female fertility, causing damage to ovarian function and disturbing follicle development. However, the effects of cigarette toxicants on ovarian function depend on duration and intensity of exposure. The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice.

Methods

Ten female Swiss mice aged 35 days were exposed to tobacco smoke from 3R4F reference research cigarettes. They were exposed to an automatic smoking machine 8 h/day, 7 days/week for 15 days. Ten age-matched controls were kept in a different room and exposed to ambient air. At the end of 15 days, five mice in each group were euthanized and the ovaries were analyzed for follicular morphometry and granulosa cell count. The remaining animals were kept for an additional 30 days for further analysis as an ex-smoker group and control group. Comparison between the two groups was evaluated by the Student’s t-test or a two-way ANOVA followed by Bonferroni post-test was applied for multiple comparisons.

Results

We found that cigarette smoke impaired antral follicular growth even after exposure cessation (p < 0.001). Both smoking and ex-smoking groups exhibited similar follicle diameter. However, at the same follicular stage, the number of granulosa cells was smaller in the ex-smoking group compared to smoking animals (p < 0.001). This was associated with increased oocyte diameter in ex-smoking animals compared to smoking animals (p < 0.01).

Conclusions

The negative effects of cigarette smoking seem to last even after exposure has been interrupted. Moreover, brief exposure during puberty may induce silent oocyte disruption, which could in turn lead to decreased fecundity rates.

【 授权许可】

   
2012 Paixão et al.; licensee BioMed Central Ltd.

【 图 表 】

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