In vitro antihelmintic effect of fifteen tropical plant extracts on excysted flukes of Fasciola hepatica

BMC Veterinary Research

Ana María García-Bores¹  José Guillermo Avila-Acevedo¹  Yolanda Vera-Montenegro³  Miguel Ángel Alonso-Díaz²  Froylán Ibarra-Velarde³  José Manuel Alvarez-Mercado³ 

[1] Lab. de Fitoquímica, UBIPRO, Facultad de Estudios Superiores Iztacala, UNAM, Avenida de los Barrios 1, Edo. de México, C.P. 54090, Mexico;Centro de Enseñanza Investigación y Extensión en Ganadería Tropical, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Km. 5.5, Carretera Federal Tlapacoyan-Martínez de la Torre, Veracruz, C.P. 93600, Mexico;Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México. Cd. Universitaria, México, C.P. 04510, DF, Mexico

关键词: In vitro;    Anthelmintic activity;    Fasciola hepatica;    Plant extracts;   

Others  :  1140556
DOI  :  10.1186/s12917-015-0362-4

 received in 2014-09-25, accepted in 2015-02-19,  发布年份 2015

【 摘 要 】

Background

Fasciolosis due to Fasciola hepatica is the most important hepatic disease in veterinary medicine. Its relevance is important because of the major economical losses to the cattle industry such as: reduction in milk, meat and wool production; miscarriages, anemia, liver condemnation and occasionally deaths, are estimated in billons of dollars.

The emergence of fluke resistance due to over or under dosing of fasciolides as well as environmental damage produced by the chemicals eliminated in field have stimulated the need for alternative methods to control Fasciola hepatica. The aim of this study was to evaluate the in vitro anthelmintic effect of fifteen tropical plant extracts used in tradicional Mexican medicine, on newly excysted flukes of Fasciola hepatica.

Results

The flukes were exposed in triplicate at 500, 250 and 125 mg/L to each extract. The efficacy was assessed as the mortality rate based on the number of live and dead flukes after 24, 48 and 72 h post-exposure. The plants with anthelmintic effect were evaluated once again with a concentration of 375 mg/L in order to confirm the results and to calculate lethal concentrations at 50%, 90% and 99% (LC₅₀, LC₉₀, and LC₉₉). Plant extracts of Lantana camara, Bocconia frutescens, Piper auritum, Artemisia mexicana and Cajanus cajan had an in vitro anthelmintic effect (P <0.05). The LC₅₀, LC₉₀ and LC₉₉ to A. mexicana, C. cajan and B. frutescens were 92.85, 210.44 and 410.04 mg/L, 382.73, 570.09 and 788.9 mg/L and 369.96, 529.94 and 710.34 mg/L, respectively.

Conclusion

It is concluded that five tropical plant extracts had promising anthelmintic effects against F. hepatica. Further studies on toxicity and in vivo biological evaluation in ruminant models might help to determine the anthelmintic potential of these plant extracts.

【 授权许可】

   
2015 Alvarez-Mercado et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]OPS (Organización Panamericana de la Salud): Zoonosis y enfermedades transmisibles comunes al hombre y a los animales. OPS, USA; 2003.
• [2]FAO. (Food and Agriculture Organization of the United Nations): Resistencia a los antiparasitarios. Estado actual con énfasis en América Latina. FAO, Italy; 2003.
• [3]Ceballos L, Moreno L, Alvarez L, Shaw L, Fairweather I, Lanusse C: Unchanged triclabendazole kinetics after co-administration with ivermectin and methimazole: failure of its therapeutic activity against triclabendazole-resistant liver fluke. BMC Vet Res 2010, 6:1-8. BioMed Central Full Text
• [4]Olaechea F, Lovera V, Larroza M, Raffo F, Cabrera R: Resistance of Fasciola hepatica against triclabendazole in cattle in Patagonia (Argentina). Vet Parasitol 2011, 178:364-6.
• [5]Anthony JP, Fyfe L, Smith H: Plant active components – a resource for antiparasitic agents? Trends Parasitol 2005, 21:462-8.
• [6]Vera-Montenegro Y, Ibarra-Velarde F, Ramirez-Avila G, Munguia-Xochihua J: In vitro fasciolicide activity of some plant extracts against newly excysted flukes. Ann NY Acad Sci 2008, 1149:180-2.
• [7]Singh TU, Kumar D, Tandan SK, Mishra KS: Inhibitory effect of essential oils of Allium sativum and Piper longumon spontaneous muscular activity of liver fluke, Fasciola gigantica. Exp Parasitol 2009, 123:302-8.
• [8]Ferreira JFS, Peaden P, Keiser J: In vitro trematocidal effects of crude alcoholic extracts of Artemisia annua, A. absinthium, Asimina triloba, and Fumaria officinalis: trematocidal plant alcoholic extracts. Parasitol 2011, 109:585-1592.
• [9]INEGI (Instituto Nacional de Estadística, Geografía e Información): Boletín de información oportuna del sector agropecuario. Instituto Nacional de Estadística, Geografía e Informática, Aguascalientes; 2010.
• [10]Cano ALM: Flora Medicinal de Veracruz I. Inventario Etnobotánico. Universidad Veracruzana, México; 1997.
• [11]Columba M: Plantas medicinales utilizadas en el estado de Morelos. Universidad Autónoma del estado de Morelos, México; 2007.
• [12]Rodríguez A, Coombes J, Jimenez R: Plantas silvestres de Puebla: herbario y jardín botánico BUAP. Herbario BUAP, México; 2009.
• [13]Fernandes FF, Freitas SEP: Acaricidal activity of an oleoresinous extract of Copaifera reticulata (Leguminosae: Caesalpinioideae) against larvae of the southern cattle tick, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Vet Parasitol 2007, 147:150-4.
• [14]Camurça-Vasconcelos ALF, Bevilaqua CML, Morais SM, Maciel MV, Costa CTC, Macedo ITF, et al.: Anthelmintic activity of Lippia sidoides essential oil on sheep gastrointestinal nematodes. Vet Parasitol 2008, 154:167-70.
• [15]Alonso-Díaz MA, Torres-Acosta JFJ, Sandoval-Castro CA, Hoste H: Comparing the sensitivity of two in vitro assays to evaluate the anthelmintic activity of tannin rich plant extracts against Haemonchus contortus. Vet Parasitol 2011, 181:360-4.
• [16]Harnborne JB: Phytochemical phylogeny; proceedings of the Phytochemical Society Symposium. Academic, London; 1970.
• [17]Trease GE: Tratado de farmacognosis. Interamericana, México; 1987.
• [18]Ibarra OF, Jenkins DC: An in vitro screen for new fasciolicidal agents. Z Parasitenkd 1984, 70:655-61.
• [19]Ibarra-Moreno S, Ibarra-Velarde F, Ávila-Acevedo JG: In vitro evaluation of anthelmintic activity with hexane, methanol and ethyl acetate with extracts processed and obtained from some mexican plants used in traditional medicine based on ethno Botanical Studies. Am. J. Plant 2012, 3:506-511.
• [20]Evans WC: Trease and Evans’ Pharmacognosy. 15th edition. Saunders Ltd, Edinburgh, U.K; 2002.
• [21]Sampietro DA, Sgariglia M, Soberón J, Quiroga E, Vattuone M: (2009) Colorimetric reactions. In Isolation, Identification and Characterization of Allelochemicals/Natural Products. Edited by Sampietro D, Catalan C, Vattuone M. CRC Press, U.S.A; 2009:73-101.
• [22]Statgraphics Centurion XVI (Computer program) Statpoint Technologies, INC. version 16.1.17(64-bits) EUA, 2011.
• [23]LeOra Software In: ROBERTSON JL, PREISLER HK, RUSSELL RM. (Eds.) A user’s guide to Probit or Logic Analysis. Berkley, USA; 2003.
• [24]Jeyathilakan N, Murali k, Anandaraj A, Latha BR, Abdul Basith S: Anthelmintic activity of essential oils of Cymbopogan nardus and Azadirachta indica on Fasciola gigantica. Tamilnadu J Vet Anim Sci 2010, 6(5):204-9.
• [25]Jeyathilakan N, Murali K, Anandaraj A, Abdul-Basith S: In vitro evaluation of anthelmintic property of ethno-veterinary plant extracts against the liver fluke Fasciola gigantica. J Parasit Dis 2012, 36(1):26-30.
• [26]Elango G, Rahuman AA: Evaluation of medicinal plant extracts against ticks and fluke. Parasitol Res 2011, 108(3):513-19.
• [27]Ibarra-Moreno S, Ibarra-Velarde F, Ávila-Acevedo JG: Obtaining the minimum lethal dose against Fasciola hepatica in vitro using plant extract hexanes with anthelmintic activity and toxicity evaluation on CD1 male mice. Am J Plant Sci 2012, 3:899-903.
• [28]Ghanem MTM, Radwan HMA, Mahdy ESM, Elkholy YM, Hassanein HD, Shahat AA: Phenolic compounds from Foeniculum vulgare (Subsp. Pipertum) (Apiaceae) herb and evaluation of hepatoprotective antioxidant activity. Phcog Res 2012, 4:104-8.
• [29]Estrada-Reyes R, Martínez-Laurrabaquio A, Ubaldo Suárez D, Araujo-Escalona AG: Neuropharmacological studies of Piper auritum Kunth (Piperaceae): antinociceptive and anxiolytic-like effects. J Med Plants Res 2013, 7(23):1718-29.
• [30]Kundu R, Dasgupta S, Biswas A, Bhattacharya A, Pal BC, Bandyopadhyay D, et al.: Cajanus cajan Linn. (Leguminosae) prevents alcohol-induced rat liver damage and augments cytoprotective function. J Ethnopharmacol 2008, 118:440-7.
• [31]Ghisalberti EL: Review Lantana camara L (Verbenaceae). Fitoterapia 2000, 71:467-86.
• [32]Hoste H, Jackson F, Athanasiadou S, Thamsborg SM, Hoskin SO: The effects of tannin-rich plants on parasitic nematodes in rumiants. Trends Parasitol 2006, 6:253-61.
• [33]Athanasiadou S, Githiori J, Kyriazakis I: Medicinal plants for helminth parasite control: facts and fiction. Animal 2007, 1:1392-400.
• [34]Calzada F, Yépez-Mulia L, Tapia-Contreras A: Effect of Mexican medicinal plant used to treat trichomoniasis on Trichomonas vaginalis trophozoites. J Ethnopharmacol 2007, 113:248-51.
• [35]Von son-de Fernex E, Alonso-Díaz MA, Valles-De la Mora B, Capetillo-Leal CM: In vitro anthelmintic activity of five tropical legumes on the exsheathment and motility of Haemonchus contortus infective larvae. Exp Parasitol 2012, 131:413-418.
• [36]Ntalli NG, Caboni P: Botanical Nematicides: A Review. J Agric Food Chem 2012, 60:9929-40.
• [37]Montgomery CT, Cassels B, Maurice S: The Rhoeadine Alkaloids. J Nat Prod 1983, 46:441-53.
• [38]Parmar VS, Jain SC, Bisht KS, Jain R, Taneja P, Jha A, et al.: Phytochemistry of the genus piper. Phytochemistry 1997, 46:597-673.
• [39]Siddiqui BS, Raza SM, Begum S, Siddiqui S, Firdous S: Pentacyclic triterpenoids from Lantana camara. Phytochemistry 1995, 38:681-5.
• [40]Akihisa T, Nishimura Y, Nakamura N, Roy K, Ghosh P, Thakur S, et al.: Sterols of Cajanus cajan and three other leguminosae seeds. Phytochemistry 1992, 31:1769-1768.
• [41]Mata R, Delgado G, Romo DVA: Sesquiterpene lactones of Artemisia mexicana var. Angustifolia. Phytochemistry 1984, 23:1665-8.