Expression of the erythropoietin receptor by germline-derived cells - further support for a potential developmental link between the germline and hematopoiesis

Journal of Ovarian Research

Mariusz Z Ratajczak³  Magda Kucia³  Sham S Kakar²  Krzysztof Marycz¹  Janina Ratajczak³  Pranesh Gunjal²  Agata Poniewierska-Baran³  Malwina Suszynska³ 

[1] University of Environmental and Life Sciences, Wroclaw, Poland;Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, KY 40202, USA;Department of Physiology, Pomeranian Medical University, Szczecin, Poland

关键词: Ovarian cancer;    VSELs;    Cancer development;    Germ line;    EpoR;   

Others  :  800969
DOI  :  10.1186/1757-2215-7-66

 received in 2014-05-03, accepted in 2014-06-11,  发布年份 2014

【 摘 要 】

Background

Expressing several markers of migrating primordial germ cells (PGCs), the rare population of quiescent, bone marrow (BM)-residing very small embryonic-like stem cells (VSELs) can be specified like PGCs into hematopoietic stem/progenitor cells (HSPCs). These two properties of VSELs support the possibility of a developmental origin of HSPCs from migrating PGCs.

Methods

To address a potential link between VSELs and germ line cells we analyzed by RT-PCR and FACS expression of erythropoietin receptor (EpoR) on murine bone marrow- and human umbilical cord blood-derived VSELs, murine and human teratocarcinoma cell lines and human ovarian cancer cells. A proper gating strategy and immunostaining excluded from FACS analysis potential contamination by erythroblasts. Furthermore, the transwell chemotaxis assays as well as adhesion and signaling studies were performed to demonstrate functionality of erythropoietin - EpoR axes on these cells.

Results

We report here that murine and human VSELs as well as murine and human teratocarcinoma cell lines and ovarian cancer cell lines share a functional EpoR.

Conclusions

Our data provide more evidence of a potential developmental link between germline cells, VSELs, and HSCs and sheds more light on the developmental hierarchy of the stem cell compartment in adult tissues.

【 授权许可】

   
2014 Suszynska et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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