Novel bacteriophage therapy for controlling metallo-beta-lactamase producing Pseudomonas aeruginosa infection in Catfish

BMC Veterinary Research

Waman N Paunikar²  Swapnil G Sanmukh²  Rajshree H Chandekar²  Mahendra P Raut¹  Krishna Khairnar² 

[1]Departments of Chemical and Biological Engineering, ChELSI, University Of Sheffield, Office No: D72, Sir Robert Hadfield Building, Mappin Street, Sheffield S1 3JD, UK

[2]Environmental Virology Cell, Council for Scientific and Industrial Research - National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440020, Maharashtra, India

关键词: Catfish;    Bacteriophage therapy;    Metallo-β-lactamase;    Multi drug resistance;    P. aeruginosa;   

Others  :  1119374
DOI  :  10.1186/1746-6148-9-264

 received in 2013-06-04, accepted in 2013-12-23,  发布年份 2013

【 摘 要 】

Background

The bacteriophage therapy is an effective antimicrobial approach with potentially important applications in medicine and biotechnology which can be seen as an additional string in the bow. Emerging drug resistant bacteria in aquaculture industry due to unrestricted use of antibiotics warrants more sustainable and environmental friendly strategies for controlling fish infections.

The isolated bacteria from fish lesions was characterised based on isolation on selective and differential medium like Pseudomonas agar, gram staining, biochemical tests and 16SrRNA sequencing. The metallo-beta-lactamase (MBL) producing bacterial isolate was evaluated using Imipenem - Ethylenediaminetetraacetic acid (EDTA) disk method. The specific bacteriophage was isolated and concentrated using coal bed developed in our lab at CSIR-NEERI. The isolated and enriched bacteriophage was characterised by nucleotide sequencing and electron microscopy. The phage therapy was applied for treating ulcerative lesion in fish.

Results

The pathogenic bacterium responsible for causing ulcerative lesions in catfish species (Clarias gariepinus) was identified as Pseudomonas aeruginosa. One out of twenty P. aeruginosa isolate showing multi drug resistance (MDR) was incidentally found to be MBL producing as determined by Imipenem-EDTA disk method. The phage therapy effectively cured the ulcerative lesions of the infected fish in 8–10 days of treatment, with a sevenfold reduction of the lesion with untreated infection control.

Conclusion

Bacteriophage therapy can have potential applications soon as an alternative or as a complement to antibiotic treatment in the aquaculture. We present bacteriophage therapy as a treatment method for controlling MDR P. aeruginosa infection in C. gariepinus. To the best of our knowledge this is a first report of application of phage therapy against MBL producing P. aeruginosa isolated from aquatic ecosystem.

【 授权许可】

   
2013 Khairnar et al.; licensee BioMed Central Ltd.

【 图 表 】

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