Molecular-based detection of the gastrointestinal pathogen Campylobacter ureolyticus in unpasteurized milk samples from two cattle farms in Ireland

Gut Pathogens

Roy D Sleator²  Gerard D Corcoran¹  Susan Bullman²  Brigid Lucey¹  Monika Koziel² 

[1]Department of Microbiology, Cork University Hospital, Wilton, Cork, Ireland

[2]Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, Ireland

关键词: Dairy;    Reservoir;    Food chain;    Emerging pathogen;    Campylobacter;   

Others  :  821870
DOI  :  10.1186/1757-4749-4-14

 received in 2012-10-18, accepted in 2012-11-04,  发布年份 2012

【 摘 要 】

Campylobacter jejuni and coli are collectively regarded as the most prevalent cause of bacterial foodborne illness worldwide. An emerging species, Campylobacter ureolyticus has recently been detected in patients with gastroenteritis, however, the source of this organism has, until now, remained unclear. Herein, we describe the molecular-based detection of this pathogen in bovine faeces (1/20) and unpasteurized milk (6/47) but not in poultry (chicken wings and caeca). This is, to the best of our knowledge, the first report of the presence of this potential gastrointestinal pathogen in an animal source, possibly suggesting a route for its transmission to humans.

【 授权许可】

   
2012 Sleator; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Moore JE, Corcoran D, Dooley JS, Fanning S, Lucey B, Matsuda M, et al.: Campylobacter. Vet Res 2005, 36:351-382.
• [2]Man SM: The clinical importance of emerging Campylobacter species. Nat Rev Gastroenterol Hepatol 2011, 8:669-685.
• [3]Bullman S, Corcoran D, O'Leary J, O'Hare D, Lucey B, Sleator RD: Emerging dynamics of human campylobacteriosis in Southern Ireland. FEMS Immunol Med Microbiol 2011, 63:248-253.
• [4]Whyte P, McGill K, Kelly L, Cowley D, Fanning S, Collins D, et al.: A Comparative Study of Thermophilic Campylobacter Isolates of Clinical, Food and Pet Origin. Safe food, Ireland: Food Safety Promotions Board; 2006.
• [5]Bullman S, Lucey B, Sleator RD: Molecular diagnostics: the changing culture of medical microbiology. Bioeng Bugs 2012, 3:1-7.
• [6]Bullman S, Corcoran D, O'Leary J, Lucey B, Byrne D, Sleator RD: Campylobacter ureolyticus: an emerging gastrointestinal pathogen? FEMS Immunol Med Microbiol 2011, 61:228-230.
• [7]Zhang L, Man SM, Day AS, Leach ST, Lemberg DA, Dutt S, et al.: Detection and isolation of Campylobacter species other than C. jejuni from children with Crohn's disease. J Clin Microbiol 2009, 47:453-455.
• [8]Mukhopadhy I, Thomson JM, Hansen R, Berry SH, El-Omar EM, Hold GL: Detection of Campylobacter concisus and other Campylobacter species in colonic biopsies from adults with ulcerative colitis. PLoS One 2011, 6:e21490.
• [9]Burgos-Portugal JA, Kaakoush NO, Raftery MJ, Mitchell HM: Pathogenic potential of Campylobacter ureolyticus. Infect Immun 2012, 80:883-890.
• [10]O'Callaghan I, Corcoran D, Lucey B: Design of a multiplex PCR assay for the simultaneous detection and confirmation of Neisseria gonorrhoeae. J Clin Pathol 2010, 63:431-433.
• [11]Wilson DJ, Gabriel E, Leatherbarrow AJ, Cheesbrough J, Gee S, Bolton E, et al.: Tracing the source of campylobacteriosis. PLoS Genet 2008, 4:e1000203.