Involvement of CSE/ H2S in high glucose induced aberrant secretion of adipokines in 3T3-L1 adipocytes

Lipids in Health and Disease

Qingbo Guan⁵  Xiangdong Wang²  Jicui Chen²  Yuchao Zhang¹  Chunxiao Yu⁵  Yuantao Liu⁴  Hanbo Wang³  Zhe Pan⁵ 

[1] Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University; National Research Center for Assisted Reproductive Technology and Reproductive Genetics; Key laboratory for Reproductive Endocrinology of Ministry of Education; Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, China250012;Department of Cell Biology, Shandong University School of Medicine, Jinan, China250012;Minimally Invasive Urology Center, Shandong Provincial Hospital affiliated to Shandong University, Jinan, China250021;Department of Endocrinology, the Second Hospital of Shandong University, Jinan, China250033;Department of Endocrinology, Shandong Provincial Hospital affiliated to Shandong University; Shandong Clinical Medical Center of Endocrinology and Metabolism; Institute of Endocrinology and Metabolism; Shandong Academy of Clinical Medicine, 324 Jing 5 Rd, Jinan, Shandong 250021, P. R. China

关键词: Adipocyte;    Adipokine;    H₂S;    Cystathionine-γ-lyase;    High glucose;   

Others  :  1151827
DOI  :  10.1186/1476-511X-13-155

 received in 2014-06-29, accepted in 2014-09-28,  发布年份 2014

【 摘 要 】

Background

Deregulated secretion of adipokines contributes to subclinical systemic inflammation associated with type 2 diabetes mellitus (T2DM). However, the mechanisms underlying are not fully understood. Hydrogen sulfide (H₂S), as an endogenous gasotransmitter, possesses an anti-inflammation activity. The aim of this study was to examine the possible involvement of H₂S in high glucose induced adipokine secretion in 3T3-L1 adipocytes.

Methods

The expression of cystathionine-gamma-lyase (CSE), the H₂S-forming enzyme, was evaluated by Western-blotting and real-time PCR. The secretion of TNF-α, MCP-1 and adiponectin was determined by radioimmunoassay and enzyme-linked immunosorbent assay (ELISA), respectively. Lentiviral empty vector and vector expressing mouse CSE were used for in vitro transduction.

Results

High glucose (HG) significantly decreased CSE expression at both protein and mRNA levels in mature 3T3-L1 adipocytes. In parallel, HG significantly increased secretion of MCP-1 while decreasing secretion of adiponectin, but had no effect on secretion of TNF-α. HG induced changes in MCP-1 and adiponectin secretion were partly attenuated by forced expression of CSE or sodium hydrosulfide (NaHS), a source of exogenous H₂S.

Conclusion

High glucose induces aberrant secretion of adipokines in mature 3T3-L1 adipocytes, favoring inflammation. The mechanism is partly related to inhibition of CSE/ H2S system.

【 授权许可】

   
2014 Pan et al.; licensee BioMed Central Ltd.

【 图 表 】

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