The role of beneficial bacteria wall elasticity in regulating innate immune response

EPMA Journal

Mykola Ya Spivak⁶  Rostyslav V. Bubnov⁵  Diana DiGioia²  Bruno Biavati²  Nadiya V. Boyko¹  Yulia O. Melnichenko¹  Olga M. Demchenko⁶  Petro M. Lytvyn³  Lidia P. Babenko¹  Liubov M. Sichel⁴  Liudmyla M. Lazarenko¹  Viktoria V. Мokrozub¹ 

[1] Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154, Zabolotny st., Kyiv D03680, Ukraine;Dipartimento di Scienze Agrarie, Alma Mater Studiorum—Bologna University, Bologna 40127, Italy;Lashkaryov Institute of Semiconductor Physics, National Academy of Sciences of Ukraine, 41, pr. Nauky, Kyiv 03028, Ukraine;Pure Research Products, LLC, 6107, Chelsea Manor Court, Boulder 80301, CO, USA;Clinical Hospital “Pheophania” of State Affairs Department, Zabolotny str., 21, Kyiv 03680, Ukraine;LCL «Diaprof», Svitlycky Str., 35, Kyiv 04123, Ukraine

关键词: Pattern-recognition receptors;    Fecal microbiota transplantation;    Nutrition;    Healthy diet;    Dietary biomarkers;    Immune disorders;    Cancer;    Microbiome;    Gut–brain axis;    Atomic force microscopy;    Cell wall elasticity;    Macrophages;    nitrosative stress;    Oxidative;    Cytokines;    Bifidobacterium;    Lactobacillus;    Gut microbiota;    and personalized medicine;    preventive;    Predictive;   

Others  :  1219083
DOI  :  10.1186/s13167-015-0035-1

 received in 2014-12-19, accepted in 2015-05-22,  发布年份 2015

【 摘 要 】

Background

Probiotics have great potential to contribute to development of healthy dietary regimes, preventive care, and an integrated approach to immunity-related disease management. The bacterial wall is a dynamic entity, depending on many components and playing an essential role in modulating immune response. The impact of cell wall elasticity on the beneficial effects of probiotic strains has not been sufficiently studied.

The aim was to investigate the effect of lactic acid bacteria (LAB) and bifidobacteria strains on phagocytic system cells (macrophages) as related to bacterial wall elasticity, estimated using atomic force microscopy (AFM).

Methods

We conducted studies on Balb/c line mice 18–20 g in weight using lyophilized strains of LAB—Lactobacillus acidophilus IMV B-7279, Lactobacillus casei IMV B-7280, Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281, and bifidobacteria—Bifidobacterium animalis VKL and Bifidobacterium animalis VKB. We cultivated the macrophages obtained from the peritoneal cavity of mice individually with the strains of LAB and bifidobacteria and evaluated their effect on macrophages, oxygen-dependent bactericidal activity, nitric oxide production, and immunoregulatory cytokines. We used AFM scanning to estimate bacterial cell wall elasticity.

Results

All strains had a stimulating effect on the functional activity of macrophages and ability to produce NO/NO ₂in vitro. Lactobacilli strains increased the production of IL-12 and IFN-γ in vitro. The AFM demonstrated different cell wall elasticity levels in various strains of LAB and bifidobacteria. The rigidity of the cell walls among lactobacilli was distributed as follows: Lactobacillus acidophilus IMV B-7279 > Lactobacillus casei IMV B-7280 > Lactobacillus delbrueckii subsp. bulgaricus IMV B-7281; among the strains of bifidobacteria: B. animalis VKB > B. animalis VKL. Probiotic strain survival in the macrophages depended on the bacterial cell wall elasticity and on the time of their joint cultivation.

Conclusion

LAB and bifidobacteria strains stimulate immune-modulatory cytokines and active oxygen and nitrogen oxide compound production in macrophages. Strains with a more elastic cell wall according to AFM data demonstrated higher resistance to intracellular digestion in macrophages and higher level of their activation.

AFM might be considered as a fast and accurate method to assess parameters of probiotic strain cell wall to predict their immune-modulatory properties.

【 授权许可】

   
2015 Mokrozub et al.

【 图 表 】

Fig. 1.

Fig. 2.

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