Changes in the cerebrospinal fluid circulatory system of the developing rat: quantitative volumetric analysis and effect on blood-CSF permeability interpretation

Fluids and Barriers of the CNS

Nathalie Strazielle²  Sandrine Blondel³  Anaïd Babikian³  Jean-François Ghersi-Egea¹ 

[1] Oncoflam Team, INSERM U1028, CNRS UMR5292, Lyon Neuroscience Research Center, Lyon, France;Brain-i, Lyon, France;BIP Platform, Faculté de Médecine RTH Laennec, INSERM U 1028, CNRS UMR5292, Lyon Neuroscience Research Center, Rue Guillaume Paradin, Cedex 08, Lyon, 69372, France

关键词: Postnatal development;    Embryo;    Claudin;    Meninges;    Blood–brain barrier;    Influx constant;    Subarachnoid space;    Ventricle;   

Others  :  1138336
DOI  :  10.1186/s12987-015-0001-2

 received in 2014-12-09, accepted in 2015-01-30,  发布年份 2015

【 摘 要 】

Background

The cerebrospinal fluid (CSF) circulatory system is involved in neuroimmune regulation, cerebral detoxification, and delivery of various endogenous and exogenous substances. In conjunction with the choroid plexuses, which form the main barrier site between blood and CSF, this fluid participates in controlling the environment of the developing brain. The lack of comprehensive data on developmental changes in CSF volume and distribution impairs our understanding of CSF contribution to brain development, and limits the interpretation of blood-CSF permeability data. To address these issues, we describe the evolution of the CSF circulatory system during the perinatal period and have quantified the volume of the different ventricular, cisternal and subarachnoid CSF compartments at three ages in developing rats.

Methods

Immunohistofluorescence was used to visualize tight junctions in parenchymal and meningeal vessels, and in choroid plexus epithelium of 19-day fetal rats. A quantitative method based on serial sectioning of frozen head and surface measurements at the cutting plane was used to determine the volume of twenty different CSF compartments in rat brain on embryonic day 19 (E19), and postnatal days 2 (P2) and 9 (P9). Blood-CSF permeability constants for sucrose were established at P2 and P9, following CSF sampling from the cisterna magna.

Results

Claudin-1 and claudin-5 immunohistofluorescence labeling illustrated the barrier phenotype acquired by all blood–brain and blood-CSF interfaces throughout the entire CNS in E19 rats. This should ensure that brain fluid composition is regulated and independent from plasma composition in developing brain. Analysis of the caudo-rostral profiles of CSF distribution and of the volume of twenty CSF compartments indicated that the CSF-to-cranial cavity volume ratio decreases from 30% at E19 to 10% at P9. CSF compartmentalization within the brain changes during this period, with a major decrease in CSF-to-brain volume ratio in the caudal half of the brain. Integrating CSF volume with the measurement of permeability constants, adds to our understanding of the apparent postnatal decrease in blood-CSF permeability to sucrose.

Conclusion

Reference data on CSF compartment volumes throughout development are provided. Such data can be used to refine blood-CSF permeability constants in developing rats, and should help a better understanding of diffusion, bulk flow, and volume transmission in the developing brain.

【 授权许可】

   
2015 Ghersi-Egea et al.; licensee BioMed Central.

【 图 表 】

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Figure 2.

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