Dihydropyrimidinase-like 3 facilitates malignant behavior of gastric cancer

Journal of Experimental & Clinical Cancer Research

Yasuhiro Kodera¹  Michitaka Fujiwara¹  Masahiko Koike¹  Hiroyuki Sugimoto¹  Goro Nakayama¹  Tsutomu Fujii¹  Suguru Yamada¹  Chie Tanaka¹  Daisuke Kobayashi¹  Ryoji Hashimoto¹  Soki Hibino¹  Hideki Takami¹  Dai Shimizu¹  Hisaharu Oya¹  Shuji Nomoto¹  Mitsuro Kanda¹ 

[1] Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan

关键词: Biomarker;    Prognosis;    Expression;    Dihydropyrimidinase-like 3;    Gastric cancer;   

Others  :  1136171
DOI  :  10.1186/s13046-014-0066-9

 received in 2014-05-17, accepted in 2014-07-24,  发布年份 2014

【 摘 要 】

Background

Gastric cancer (GC) remains to have a poor prognosis via diverse process of cancer progression. Dihydropyrimidinase-like 3 (DPYSL3) is a cell adhesion molecule that has been reported to be involved in the metastatic process of tumor cells. The aim of this study was to identify a novel clinically-relevant biomarker of GC.

Methods

Expression analysis of DPYSL3 mRNA and protein levels was conducted using GC cell lines and 238 pairs of surgically resected gastric tissues. Correlations between expression status of DPYSL3 and clinicopathological parameters were investigated.

Results

DPYSL3 mRNA expression levels positively correlated with those of potentially interacting genes (VEGF, FAK and EZR) in GC cell lines. GC tissues from tumors with distant metastases (stage IV cancer) showed elevated expression levels of DPYSL3 mRNA. The DPYSL3 staining intensity in immunochemical staining was consistent with the mRNA expression patterns in GC tissues. High DPYSL3 mRNA expression in GCs was significantly associated with more malignant phenotypes and was an independent prognostic factor. Moreover, patients with high DPYSL3 mRNA expression had a significantly shorter recurrence free survival after curative resection. In subgroup analysis based on tumor histology, similar tendency was observed between patients with differentiated and undifferentiated GCs.

Conclusions

Expression status of DPYSL3 in GC tissues may represent a promising biomarker for the malignant behavior of GC.

【 授权许可】

   
2014 Kanda et al.; BioMed Central Ltd

【 图 表 】

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