Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest

Genome Biology

Joerg Bohlmann⁴  Steven JM Jones⁶  Inanc Birol¹  Dezene P W Huber⁵  Felix AH Sperling³  Richard Moore²  Pawan Pandoh²  Yongjun Zhao²  Jasmine K Janes³  Hannah Henderson⁴  Maria Li⁴  Anh Nguyen⁴  Shaun D Jackman²  Diana L Palmquist²  Greg A Taylor²  Simon K Chan²  T Roderick Docking²  Nancy Y Liao²  Macaire MS Yuen⁴  Christopher I Keeling⁴ 

[1] Department of Medical Genetics, University of British Columbia, University of British Columbia, 4500 Oak St., Vancouver, BC, Canada V6H 3N1;Canada's Michael Smith Genome Sciences Centre, 570 W 7th Ave #100 Vancouver, BC, Canada V5Z 4S6;Department of Biological Sciences, CW 405, Biological Sciences Bldg., University of Alberta, Edmonton, AB, Canada T6G 2E9;Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall, Vancouver, BC, Canada V6T 1A4;Ecosystem Science and Management Program, University of Northern British Columbia, 3333 University Way, Prince George, BC, Canada V2N 4Z9;Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada V5A 1S6

关键词: sex chromosomes;    horizontal gene transfer;    plant cell wall-degrading enzymes;    glutathione S-transferase;    cytochrome P450;    conifer;    bark beetles;    Scolytinae;    Curculionoidea;    Coleoptera;   

Others  :  866874
DOI  :  10.1186/gb-2013-14-3-r27

 received in 2012-10-10, accepted in 2013-03-27,  发布年份 2013

【 摘 要 】

Background

The mountain pine beetle, Dendroctonus ponderosae Hopkins, is the most serious insect pest of western North American pine forests. A recent outbreak destroyed more than 15 million hectares of pine forests, with major environmental effects on forest health, and economic effects on the forest industry. The outbreak has in part been driven by climate change, and will contribute to increased carbon emissions through decaying forests.

Results

We developed a genome sequence resource for the mountain pine beetle to better understand the unique aspects of this insect's biology. A draft de novo genome sequence was assembled from paired-end, short-read sequences from an individual field-collected male pupa, and scaffolded using mate-paired, short-read genomic sequences from pooled field-collected pupae, paired-end short-insert whole-transcriptome shotgun sequencing reads of mRNA from adult beetle tissues, and paired-end Sanger EST sequences from various life stages. We describe the cytochrome P450, glutathione S-transferase, and plant cell wall-degrading enzyme gene families important to the survival of the mountain pine beetle in its harsh and nutrient-poor host environment, and examine genome-wide single-nucleotide polymorphism variation. A horizontally transferred bacterial sucrose-6-phosphate hydrolase was evident in the genome, and its tissue-specific transcription suggests a functional role for this beetle.

Conclusions

Despite Coleoptera being the largest insect order with over 400,000 described species, including many agricultural and forest pest species, this is only the second genome sequence reported in Coleoptera, and will provide an important resource for the Curculionoidea and other insects.

【 授权许可】

   
2013 Keeling et al.; licensee BioMed Central Ltd.

【 图 表 】

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