Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams

BMC Veterinary Research

Renato L Santos¹  Tatiane A Paixão²  Érica A Costa¹  Custódio A Carvalho¹  Mariana N Xavier¹  Luciana F Costa¹  Teane MA Silva¹  Valéria S Moustacas¹ 

[1] Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG Brazil;Departamento de Patologia Geral, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG 31270-901, Brazil

关键词: Multiplex polymerase chain reaction;    Epididymitis;    Histophilus somni;    Brucella ovis;    Actinobacillus seminis;   

Others  :  1119587
DOI  :  10.1186/1746-6148-9-51

 received in 2013-01-26, accepted in 2013-03-18,  发布年份 2013

【 摘 要 】

Background

Infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. The most common causative agents of these infections are Brucella ovis, Actinobacillus seminis, and Histophilus somni. The aim of this study was to develop a multiplex PCR assay for simultaneous detection of Brucella ovis, Actinobacillus seminis, and Histophilus somni with species-specific primers applied to biological samples for molecular diagnosis of these infections.

Results

The multiplex assay was capable of detecting B. ovis, A. seminis, and H. somni DNA simultaneously from genomic bacterial DNA samples and pool of semen samples from experimentally infected rams. The method was highly specific since it did not amplify DNA from other bacterial species that can potentially cause epididymitis in rams as well as species phylogenetically related to B. ovis. All negative control samples were negative in PCR multiplex assay. Urine can be used as an alternative to semen samples.

Conclusions

The species-specific multiplex PCR assay developed in this study can be successfully used for the detection of three of the most common bacterial causes of ovine epididymitis.

【 授权许可】

   
2013 Moustacas et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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