An experimental protocol for mimicking pathomechanisms of traumatic brain injury in mice

Experimental & Translational Stroke Medicine

Anna-Leena Sirén³  Christoph Kleinschnitz¹  Furat Raslan³  Csanád Várrallyay²  Christiane Albert-Weißenberger¹ 

[1] University of Würzburg, Department of Neurology, Würzburg, Germany;University of Würzburg, Department of Neuroradiology Würzburg, Germany;University of Würzburg, Department of Neurosurgery, Würzburg, Germany

关键词: neurons;    microglia;    astrocyte;    neurobehavioural deficits;    traumatic brain injury;    closed head injury;   

Others  :  861979
DOI  :  10.1186/2040-7378-4-1

 received in 2012-01-30, accepted in 2012-02-02,  发布年份 2012

【 摘 要 】

Traumatic brain injury (TBI) is a result of an outside force causing immediate mechanical disruption of brain tissue and delayed pathogenic events. In order to examine injury processes associated with TBI, a number of rodent models to induce brain trauma have been described. However, none of these models covers the entire spectrum of events that might occur in TBI. Here we provide a thorough methodological description of a straightforward closed head weight drop mouse model to assess brain injuries close to the clinical conditions of human TBI.

【 授权许可】

   
2012 Albert-Weißenberger et al; licensee BioMed Central Ltd.

【 图 表 】

【 参考文献 】

• [1]Gaetz M: The neurophysiology of brain injury. Clin Neurophysiol 2004, 115:4-18.
• [2]Faul M, Xu L, Wald MM, Coronado VG: Traumatic Brain Injury in the United States: Emergency Department Visits, Hospitalizations, and Deaths, 2002-2006. In Book Traumatic Brain Injury in the United States: Emergency Department Visits, Hospitalizations, and Deaths, 2002-2006. City; 2010.
• [3]Maegele M, Engel D, Bouillon B, Lefering R, Fach H, Raum M, Buchheister B, Schaefer U, Klug N, Neugebauer E: Incidence and outcome of traumatic brain injury in an urban area in Western Europe over 10 years. Eur Surg Res 2007, 39:372-379.
• [4]Mock C, Joshipura M, Goosen J, Lormand JD, Maier R: Strengthening trauma systems globally: the Essential Trauma Care Project. J Trauma 2005, 59:1243-1246.
• [5]Yattoo G, Tabish A: The profile of head injuries and traumatic brain injury deaths in Kashmir. J Trauma Manag Outcomes 2008, 2:5. BioMed Central Full Text
• [6]Chiu WT, Huang SJ, Tsai SH, Lin JW, Tsai MD, Lin TJ, Huang WC: The impact of time, legislation, and geography on the epidemiology of traumatic brain injury. J Clin Neurosci 2007, 14:930-935.
• [7]Martins ET, Linhares MN, Sousa DS, Schroeder HK, Meinerz J, Rigo LA, Bertotti MM, Gullo J, Hohl A, Dal-Pizzol F, Walz R: Mortality in severe traumatic brain injury: a multivariated analysis of 748 Brazilian patients from Florianopolis City. J Trauma 2009, 67:85-90.
• [8]Albert-Weissenberger C, Siren AL: Experimental traumatic brain injury. Exp Transl Stroke Med 2010, 2:16. BioMed Central Full Text
• [9]Rakos G, Kis Z, Nagy D, Lur G, Farkas T, Hortobagyi T, Vecsei L, Toldi J: Evans Blue fluorescence permits the rapid visualization of non-intact cells in the perilesional rim of cold-injured rat brain. Acta Neurobiol Exp (Wars) 2007, 67:149-154.
• [10]Raslan F, Schwarz T, Meuth SG, Austinat M, Bader M, Renne T, Roosen K, Stoll G, Siren AL, Kleinschnitz C: Inhibition of bradykinin receptor B1 protects mice from focal brain injury by reducing blood-brain barrier leakage and inflammation. J Cereb Blood Flow Metab 2010, 30:1477-1486.
• [11]Stoffel M, Blau C, Reinl H, Breidt J, Gersonde K, Baethmann A, Plesnila N: Identification of brain tissue necrosis by MRI: validation by histomorphometry. J Neurotrauma 2004, 21:733-740.
• [12]Eriskat J, Furst M, Stoffel M, Baethmann A: Correlation of lesion volume and brain swelling from a focal brain trauma. Acta Neurochir Suppl 2003, 86:265-266.
• [13]Siren AL, Radyushkin K, Boretius S, Kammer D, Riechers CC, Natt O, Sargin D, Watanabe T, Sperling S, Michaelis T, et al.: Global brain atrophy after unilateral parietal lesion and its prevention by erythropoietin. Brain 2006, 129:480-489.
• [14]Flierl MA, Stahel PF, Beauchamp KM, Morgan SJ, Smith WR, Shohami E: Mouse closed head injury model induced by a weight-drop device. Nat Protoc 2009, 4:1328-1337.
• [15]Foda MA, Marmarou A: A new model of diffuse brain injury in rats. Part II: Morphological characterization. J Neurosurg 1994, 80:301-313.
• [16]Marmarou A, Foda MA, van den Brink W, Campbell J, Kita H, Demetriadou K: A new model of diffuse brain injury in rats. Part I: Pathophysiology and biomechanics. J Neurosurg 1994, 80:291-300.
• [17]Loane DJ, Pocivavsek A, Moussa CE, Thompson R, Matsuoka Y, Faden AI, Rebeck GW, Burns MP: Amyloid precursor protein secretases as therapeutic targets for traumatic brain injury. Nat Med 2009, 15:377-379.
• [18]Feeney DM, Boyeson MG, Linn RT, Murray HM, Dail WG: Responses to cortical injury: I. Methodology and local effects of contusions in the rat. Brain Res 1981, 211:67-77.
• [19]Dail WG, Feeney DM, Murray HM, Linn RT, Boyeson MG: Responses to cortical injury: II. Widespread depression of the activity of an enzyme in cortex remote from a focal injury. Brain Res 1981, 211:79-89.
• [20]Panikashvili D, Simeonidou C, Ben-Shabat S, Hanus L, Breuer A, Mechoulam R, Shohami E: An endogenous cannabinoid (2-AG) is neuroprotective after brain injury. Nature 2001, 413:527-531.
• [21]Chen Y, Constantini S, Trembovler V, Weinstock M, Shohami E: An experimental model of closed head injury in mice: pathophysiology, histopathology, and cognitive deficits. J Neurotrauma 1996, 13:557-568.
• [22]Shapira Y, Shohami E: Experimental studies on brain oedema after blunt head injury: experimental approaches from animal experimentation to actual or possible clinical application. Eur J Anaesthesiol 1993, 10:155-173.
• [23]Shukla D, Devi BI, Agrawal A: Outcome measures for traumatic brain injury. Clinical Neurology and Neurosurgery 2011, 113:435-441.
• [24]Castejon OJ: Morphological astrocytic changes in complicated human brain trauma. A light and electron microscopic study. Brain Inj 1998, 12:409-427. discussion 407
• [25]Castejon OJ: Electron microscopic analysis of cortical biopsies in patients with traumatic brain injuries and dysfunction of neurobehavioural system. J Submicrosc Cytol Pathol 1998, 30:145-156.
• [26]Oehmichen M, Meissner C: Routine techniques in forensic neuropathology as demonstrated by gunshot injury to the head. Legal Medicine 2009, 11(Supplement 1):S50-S53.
• [27]Oehmichen M, Walter T, Meissner C, Friedrich HJ: Time course of cortical hemorrhages after closed traumatic brain injury: statistical analysis of posttraumatic histomorphological alterations. J Neurotrauma 2003, 20:87-103.
• [28]Kleinschnitz C, Stoll G, Bendszus M, Schuh K, Pauer HU, Burfeind P, Renne C, Gailani D, Nieswandt B, Renne T: Targeting coagulation factor XII provides protection from pathological thrombosis in cerebral ischemia without interfering with hemostasis. J Exp Med 2006, 203:513-518.
• [29]Tsenter J, Beni-Adani L, Assaf Y, Alexandrovich AG, Trembovler V, Shohami E: Dynamic changes in the recovery after traumatic brain injury in mice: effect of injury severity on T2-weighted MRI abnormalities, and motor and cognitive functions. J Neurotrauma 2008, 25:324-333.