Association of chronic diabetes and hypertension in sural nerve morphometry: an experimental study

Diabetology & Metabolic Syndrome

Valéria Paula Sassoli Fazan⁵  Helio Cesar Salgado²  Jaci Ayrton Castania²  Milena Cardoso Maia Neubern⁴  Renata da Silva Ferreira⁴  Karina Laurenti Sato³  Marcelo Rodrigo Tavares¹  Luciana Sayuri Sanada⁴ 

[1] Department of Anatomy, José do Rosário Velano University, Alfenas, MG, Brazil;Department of Physiology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil;Departament of Physical Therapy, Federal University of Sergipe, Aracaju, SE, Brazil;Department of Neuroscience and Behavioral Neurosciences, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil;Department of Surgery and Anatomy, School of Medicine of Ribeirão Preto, USP, Av. Bandeirantes 3900, Ribeirão Preto, SP, Brazil

关键词: Streptozotocin;    Spontaneously hypertensive rats;    Experimental diabetes;    Experimental hypertension;    Morphometry;    Myelinated fibers;    Sural nerve;   

Others  :  1128667
DOI  :  10.1186/s13098-015-0005-8

 received in 2014-12-12, accepted in 2015-02-02,  发布年份 2015

【 摘 要 】

Background

Prospective studies have shown incidence rates of hypertension in diabetes mellitus to be three times that of subjects without diabetes mellitus. The reverse also applies, with the incidence of diabetes two to three times higher in patients with hypertension. Despite this common clinical association, the contribution of each isolated entity in the development of a neuropathy is still not well understood. The aims of the present study were to investigate the presence of peripheral neuropathy in spontaneously hypertensive rats (SHR) and SHR with chronically induced diabetes, using a morphological and morphometric study of the sural nerves.

Methods

Female SHR and normotensive Wistar rats (WR), 8 weeks old, received a single intravenous injection of streptozotocin (STZ) through the tail vein. Controls from both strains received vehicle. Twelve weeks after the injection, sural nerves were dissected and prepared for light microscopy. Morphometry of sural nerve fascicles and myelinated fibers was performed with the aid of computer software.

Results

The sural nerve myelinated fibers were highly affected by experimental diabetes in normotensive rats, causing mainly the reduction of the fiber size. Hypertensive rats showed characteristics of small fiber neuropathy and a severe reduction of the number and density or Schwann cells. The association between diabetes and hypertension caused an increase on the average size of the myelinated fibers, pointing to a small fiber loss, associated to axonal atrophy.

Conclusions

Our study gives morphological support to the existence of a neuropathy due to hypertension, which is among one of the most common risk factors for diabetic neuropathy. The association between the two neuropathies showed to be a complex alteration, involving and including both, large and small fibers neuropathy. Hypertension caused, indeed, an exacerbation of the alterations already observed in experimental models of diabetic neuropathy.

【 授权许可】

   
2015 Sanada et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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