Preparation of a nano emodin transfersome and study on its anti-obesity mechanism in adipose tissue of diet-induced obese rats

Journal of Translational Medicine

Xiaolian Song³  Changhui Wang³  Shen Qu³  Min Tan³  Changxing Shen³  Lei Xu³  Haiyan Lin³  Qing Yuan³  Qingqing Huang³  Jin Chao²  Xinwen Chang³  Jidong Zhang⁴  Shilong Han¹  Shuanshuan Xie³  Kun Lu³ 

[1] Department of Molecular and Biomedical Pharmacology, University of Kentucky, Lexington KY 40506, USA;Department of Chemical Sciences and Nano-biomedicine, Tongji University, Shanghai 200072, China;Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China;Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kurokami 8608555, Japan

关键词: G0S2;    ATGL;    Transfersome;    Emodin;    Nano;    Obesity;   

Others  :  817609
DOI  :  10.1186/1479-5876-12-72

 received in 2013-12-21, accepted in 2014-03-06,  发布年份 2014

【 摘 要 】

Objective

To describe the preparation of nano emodin transfersome (NET) and investigate its effect on mRNA expression of adipose triglyceride lipase (ATGL) and G0/G1 switch gene 2 (G0S2) in adipose tissue of diet-induced obese rats.

Methods

NET was prepared by film-ultrasonic dispersion method. The effects of emodin components at different ratios on encapsulation efficiency were investigated.The NET envelopment rate was determined by ultraviolet spectrophotometry. The particle size and Zeta potential of NET were evaluated by Zetasizer analyzer. Sixty male SD rats were assigned to groups randomly. After 8-week treatment, body weight, wet weight of visceral fat and the percentage of body fat (PBF) were measured. Fasting blood glucose and serum lipid levels were determined. The adipose tissue section was HE stained, and the cellular diameter and quantity of adipocytes were evaluated by light microscopy. The mRNA expression of ATGL and G0S2 from the peri-renal fat tissue was assayed by RT-PCR.

Results

The appropriate formulation was deoxycholic acid sodium salt vs. phospholipids 1:8, cholesterol vs. phospholipids 1:3, vitamin Evs. phospholipids 1:20, and emodin vs. phospholipid 1:6. Zeta potential was −15.11 mV, and the particle size was 292.2 nm. The mean encapsulation efficiency was (69.35 ± 0.25)%. Compared with the obese model group, body weight, wet weight of visceral fat, PBF and mRNA expression of G0S2 from peri-renal fat tissue were decreased significantly after NET treatment (all P < 0.05), while high-density lipoprotein cholesterol (HDL-C), the diameter of adipocytes and mRNA expression of ATGL from peri-renal fat tissue were increased significantly (all P < 0.05).

Conclusion

The preparation method is simple and reasonable. NET with negative electricity was small and uniform in particle size, with high encapsulation efficiency and stability. NET could reduce body weight and adipocyte size, and this effect was associated with the up-regulation of ATGL, down-regulation of G0S2 expression in the adipose tissue, and improved insulin sensitivity.

【 授权许可】

   
2014 Lu et al.; licensee BioMed Central Ltd.

【 图 表 】

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