Occurrence of antibiotic resistance in bacteria isolated from seawater organisms caught in Campania Region: preliminary study

BMC Veterinary Research

Aniello Anastasio²  Maria L Cortesi²  Gaetano Oliva²  Giuseppe A Martin²  Silvia Cappiello¹  Raffaele Marrone²  Giorgio Smaldone² 

[1] Departement of Veterinary Medicine and Animal Production, Veterinary pharmacovigilance center, Campania region, via F. Delpino 1, 80137 Naples, NA, Italy;Departement of Veterinary Medicine and Animal Production, University of Naples "Federico II", via F. Delpino 1, 80137 Naples, NA, Italy

关键词: Antibiotic residues;    Fish;    Vibrio strains;    Antibiotic resistance;   

Others  :  870174
DOI  :  10.1186/1746-6148-10-161

 received in 2014-01-08, accepted in 2014-06-30,  发布年份 2014

【 摘 要 】

Background

Environmental contamination by pharmaceuticals is a public health concern: drugs administered to humans and animals are excreted with urine or faeces and attend the sewage treatment. The main consequences of use and abuse of antibiotics is the development and diffusion of antibiotic resistance that has become a serious global problem. Aim of the study is to evaluate the presence of antimicrobial residues and to assess the antimicrobial resistance in bacteria species isolated from different wild caught seawater fish and fishery products.

Results

Three antibiotic substances (Oxytetracicline, Sulfamethoxazole and Trimethoprim) were detected (by screening and confirmatory methods) in Octopus vulgaris, Sepia officinalis and Thais haemastoma. All Vibrio strains isolated from fish were resistant to Vancomycin (VA) and Penicillin (P). In Vibrio alginolyticus, isolated in Octopus vulgaris, a resistance against 9 antibiotics was noted.

Conclusions

Wild caught seawater fish collected in Gulf of Salerno (Campania Region), especially in marine areas including mouths of streams, were contaminated with antibiotic-resistant bacteria strains and that they might play an important role in the spread of antibiotic-resistance.

【 授权许可】

   
2014 Smaldone et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Forth W, Henschler D, Rummel W, Starke K: Allgemeine und spezielle. In Pharmakologie und Toxikologie. 6th edition. Vienna, Zurich: Wissenschaftsverlag, Mannheim, Leipzig; 1996.
• [2]Stumpf M, Ternes T, Haberer K, Seel P, Baumann W: Nachweis von Arzneimittelrückständen in Kläranlagen und Fließgewässern. Vom Wasser 1996, 86:291-303.
• [3]Jobling S, Williams R, Johnson A, Taylor A, Gross-Sorokin M, Nolan M, Tyler C, Van Aerle R, Santos E, Brighty G: Predicted exposures to steroid estrogens in U.K. rivers correlate with widespread sexual disruption in wild fish populations. Environ Health Perspect 2006, 114(Suppl 1):32-39.
• [4]Pomati F, Castiglioni S, Zuccato E, Fanelli R, Rossetti C, Calamari D: Effects of environmental contamination by therapeutic drugs on human embryonic cells. Environ Sci Technol 2006, 40:2442-2447.
• [5]Kummerer K: Resistance in the environment. J Antimicrob Chemother 2004, 54:311-320.
• [6]Miranda JM, Vázquez BI, Fente CA, Barros-Velázquez J, Cepeda A, Franco CM: Evolution of resistance in poultry intestinal Escherichia coli during three commonly used antimicrobial therapeutic treatments in poultry. Poult Sci 2008, 87:1643-1648.
• [7]Gjerde J, Boe B: Isolation and characterization of Vibrio alginolyticus and Vibrio parahaemolyticus from the Norwegian coastal environment. Acta Vet Scand 1981, 22:331-343.
• [8]Jergensen BR, Huss HH: Growth and activity of Shewanella putrefaciens isolated from spoiling fish. Int J Food Microbiol 1989, 9:51-62.
• [9]Martinez JL: Recent advances on antibiotic resistance genes. In Recent Advances in Marine Biotechnology. Molecular Genetics of Marine Organisms, Volume 10 Edited by Fingerman N. 2003, pp13-pp32.
• [10]Randall LP, Ridley AM, Cooles SW, Sharma M, Sayers AR, Pumbwe L, Newell DG, Piddock LJV, Woodward MJ: Prevalence of multiple antibiotic resistance in 443 Campylobacter spp. isolated from humans and animals. J Antimic Chemot 2003, 52:507-510.
• [11]Carneiro LAM, Silva APS, Merquior VLC, Queiroz MLP: Antimicrobial resistance in Gram-negative bacilli isolated from infant formulas. FEMS Microbiol Lett 2003, 228:175-179.
• [12]Randall LP, Cooles SW, Osborn MK, Piddock LJV, Woodward MJ: Antibiotic resistance genes, integrons and multiple antibiotic resistance in thirty-Wve serotypes of Salmonella enterica isolated from humans and animals in the UK. J Antimicrob Chemother 2004, 53:208-216.
• [13]Ziha-Zari WI, Llanes C, Köhler T, Pechere JC, Plesiat P: In vivo emergence of multidrug-resistant mutants of Pseudomonas aeruginosa overexpressing the active eZux system MexA-MexB-OprM. Antimicrob Agents Ch 1999, 43(2):287-291.
• [14]Miranda JM, Vázquez BI, Fente CA, Barros-Velázquez J, Cepeda A, Franco Abuín CM: Antimicrobial resistance in Escherichia coli strains isolated from organic and conventional pork meat: a comparative survey. Eur Food Res Technol 2008, 226:371-375.
• [15]Schmidt AS, Bruun MS, Dalsgaard I, Pedersen K, Larsen JL: Occurrence of antimicrobial resistance in fish pathogenic and environmental bacteria associated with four Danish rainbow trout farms. Appl Environ Microb 2000, 66:4908-4915.
• [16]Bager F, Madsen M, Christensen J, Aerestrup FM: Avoparcin used as a growth promoter is associated with the occurrence of vancomycin – resistant Enterococcus faecium on Danish poultry and pig farms. Prev Vet Med 1997, 95:112.
• [17]Kerry J, Coyne R, Gilroy D, Hiney M, Smith P: Spatial distribution of oxytetracycline and elevated frequencies of oxytetracycline resistance in sediments beneath salmon farm following oxytetracycline therapy. Aquaculture 1996, 145:31-39.
• [18]Baquero F, Martinez JL, Canton R: Antibiotics and antibiotic resistance in water environments. Curr Opin Biotechnol 2008, 19:260-265.
• [19]Ternes TA: Occurrence of drugs in sewage treatment plants and rivers. In Water Res 1998, 32:3245-3260.
• [20]Iglesias A, Nebot C, Miranda JM, Vazquez BI, Cepeda A: Detection and quantitative analysis of 21 veterinary drugs in river water using high-pressure liquid chromatography coupled to tandem mass spectrometry. Environ Sci Pollut R 2012, 19:3235-3249.
• [21]Migliore L, Brambilla G, Cozzolino S, Gaudio L: Effect on plants of sulphadimethoxine used in intensive farming Panicum miliaceum, Pisum sativum and Zea mays. Agric Ecosys Environ 1995, 52:103-110.
• [22]Boehm R: Effects of residues of antiinfectives in animal excrements upon slurry management and upon soil. Dtsch tierärztl Wschr 1996, 103:264-268.
• [23]Berger K, Petersen B, Buening-Pfaue H: Persistence of drugs occurring in liquid manure in the food chain. Arch Lebensmittelhyg 1986, 37:99-102.
• [24]Joint FAO/OIE/WHO: Expert Workshop on Non-Human Antimicrobial Usage and Antimicrobial Resistance: Scientific assessment, Geneva, December 1 – 5, 2003. Databasehttp://www.who.int/foodsafety/micro/meetings/nov2003/en/ webcite
• [25]ISO/TS 21872–1:2007: Microbiology of food and animal feeding stuffs – Horizontal method for the detection of potentially enteropathogenic Vibrio spp. – Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae. Databasehttp://www.iso.org/iso/catalogue_detail.htm?csnumber=38278 webcite
• [26]ISO/TS 21872–2:2007: Microbiology of food and animal feeding stuffs -- Horizontal method for the detection of potentially enteropathogenic Vibrio spp. -- Part 2: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae. Databasehttp://www.iso.org/iso/catalogue_detail.htm?csnumber=38279 webcite
• [27]Di Pinto A, Ciccarese G, De Corato R, Novello L, Terio V: Detection of pathogenic Vibrio parahaemolyticus in southern Italian shellfish. Food Control 2008, 19:1037-1041.
• [28]Comité de l’antibiogramme de la Société Française de Microbiologie Database [http://www.sfm-microbiologie.org webcite]
• [29]Fernandez-Torres R, Bello Lopez MA, Olias Consentino M, Callejon Mochon M: Simultaneous determination of selected antibiotics and their main metabolites in fish and mussel samples by high-performance liquid chromatography with diode array-fluorescence (HPLC-DAD-FLD) detection. Anal Lett 2011, 44:2357-2372.
• [30]De Jesús Valle MJ, López FG, Navarro AS: Development and validation of an HPLC method for vancomycin and its application to a pharmacokinetic study. J Pharmaceut Biomed 2008, 48:835-839.
• [31]Rapporto 2012 sulle antibiotico resistenze e sull’uso di antibiotici rilevati nelle strutture ospedaliere della Campania Databasehttp://resources.regione.campania.it/slide/files/Assessori/sanita/OER/file_13594_GNR.pdf webcite