Multiple injections of human umbilical cord-derived mesenchymal stromal cells through the tail vein improve microcirculation and the microenvironment in a rat model of radiation myelopathy

Journal of Translational Medicine

Hua You³  Wei-Jing Zhang³  Jia-Ning Wang⁶  Zhi-Fang Li³  Yan-Qing Wang³  Yuan Liu¹  Zai-Liang Yang¹  Feng Liang³  Lei Wang⁶  Wan-Liang Sun⁷  Ke Zheng⁴  Hai-Xing Mai³  Xiu-Bin Xiao³  Jing Zhang⁵  Li Wei² 

[1] Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China;Key Laboratory of Birth Defects and Reproductive Health of the National Health and Family Planning Commission, Chongqing Population and the Family Planning Science and Technology Research Institute, Chongqing 400020, China;Affiliated Hospital of the Academy of Military Medical Sciences, No.8 East Main Street, Fengtai District, Beijing 100071, China;Department of Endocrine Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;The First Affiliated Hospital of Liaoning Medical University, No.2 Renmin Street, Guta District, Jinzhou 121000, Liaoning Province, China;Department of Cardiology & Institute of Clinical Medicine, Hubei University of Medicine, Shiyan 442000, Hubei, China;PLA 205 Hospital, No.9 Chongqing Road, Guta District, Jinzhou 121000, Liaoning Province, China

关键词: Human umbilical cord-derived mesenchymal stromal cells;    Paracrine system;    Spinal cord blood flow;    Endothelial cell;    Radiation myelopathy;   

Others  :  1148220
DOI  :  10.1186/s12967-014-0246-6

 received in 2014-07-05, accepted in 2014-08-28,  发布年份 2014

【 摘 要 】

Background

At present, no effective clinical treatment is available for the late effects of radiation myelopathy. The aim of the present study was to assess the therapeutic effects of human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) in a rat model of radiation myelopathy.

Methods

An irradiated cervical spinal cord rat model was generated. UC-MSCs were injected through the tail vein at 90, 97, 104 and 111 days post-irradiation. Behavioral tests were performed using the forelimb paralysis scoring system, and histological damage was examined using Nissl staining. The microcirculation in the spinal cord was assessed using von Willebrand factor (vWF) immunohistochemical analysis and laser-Doppler flowmetry. The microenvironment in the spinal cord was determined by measuring the pro-inflammatory cytokines interleukin-1? (IL-1?) and tumor necrosis factor-? (TNF-?) in the serum and the anti-inflammatory cytokines brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) in the spinal cord.

Results

Multiple injections of UC-MSCs through the tail veil decreased the forelimb paralysis, decreased spinal cord histological damage, increased the number of neurons in the anterior horn of the spinal cord, increased the endothelial cell density and the microvessel density in the white matter and gray matter of the spinal cord, increased the relative magnitude of spinal cord blood flow, down-regulated pro-inflammatory cytokine expression in the serum, and increased anti-inflammatory cytokine expression in the spinal cord.

Conclusion

Multiple injections of UC-MSCs via the tail vein in a rat model of radiation myelopathy significantly improved the microcirculation and microenvironment through therapeutic paracrine effects.

【 授权许可】

   
2014 Wei et al.; licensee BioMed Central Ltd.

【 图 表 】

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