Amelioration of lipid peroxidation in vivo and in vitro by Satureja khozestanica essential oil in alloxan-induced diabetic rats

Journal of Diabetes & Metabolic Disorders

Foad Abdolahpour²  Shahrokh Bagheri²  Khadijeh Beyranvand²  Maryam Hormozi²  Gholamreza Shahsavari²  Ali Khosrowbeygi²  Majid Tavafi¹  Hassan Ahmadvand² 

[1] Department of Anatomy, Faculty, Lorestan University of Medical Sciences, Khoram Abad, Iran;Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran

关键词: Essential oil;    Satureja khozestanica;    Rat;    Lipid peroxidation;    Diabetes;   

Others  :  1135805
DOI  :  10.1186/s40200-014-0119-9

 received in 2013-03-10, accepted in 2014-12-02,  发布年份 2014

【 摘 要 】

Background

We examined possible protective effect of Satureja khozestanica essential oil (SKE) on in vivo and in vitro lipid peroxidation in alloxan-induced Type 1 diabetic rats.

Methods

Thirty Sprage-dawley male rats were divided into three groups randomly; group one as control, group two diabetic untreatment, and group three treatments with SKE by 500 ppm in drinking water, respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After 8 weeks, animals were anaesthetized, livers and kidneys were then removed immediately and used fresh or kept frozen until their lipid peroxidation analysis. Lipid peroxidation was determined by measurement of thiobarbituric acid reactive substances (TBARS). Blood samples were also collected before killing to measure the levels of fasting blood suger (FBS) and lipid peroxidation.

Results

SKE significantly inhibited the levels of FBS, TBARS serum and kidney content in treated group compared with the diabetic untreated group. Also the levels of malonedialdehyde liver content unaltered in treated group. SKE significantly inhibited LDL oxidation in vitro.

Conclusions

The findings showed that SKE exerts beneficial effects on the lipid peroxidation in alloxan-induced Type 1 diabetic rats.

【 授权许可】

   
2014 Ahmadvand et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

【 参考文献 】

• [1]Najm W, Lie D: Herbals used for diabetes, obesity, and metabolic syndrome. Prim Care 2010, 37(2):237-254.
• [2]Barbosa KB, Bressan J, Zulet MA, Martínez Hernández JA: Influence of dietary intake on plasma biomarkers of oxidative stress in humans. An Sist Sanit Navar 2008, 31(3):259-280.
• [3]Lin Y, Sun Z: Current views on type 2 diabetes. J Endocrinol 2010, 204(1):1-11.
• [4]Ahmad A, Mondello S, Di Paola R, Mazzon E, Esposito E, Catania MA, Italiano D, Mondello P, Aloisi C, Cuzzocrea S: Protective effect of apocynin, a NADPH-oxidase inhibitor, against contrast-induced nephropathy in the diabetic rats: A comparison with n-acetylcysteine. Eur J Pharmacol 2012, 674(2–3):397-406.
• [5]Drissi A, Girona J, Cherki M, Godàs G, Derouiche A, El Messal M, Saile R, Kettani A, Solà R, Masana L, Adlouni A: Evidence of hypolipemiant and antioxidant properties of argan oil derived from the argan tree (Argania spinosa). Clin Nutr 2004, 3(5):1159-1166.
• [6]Giacco F, Brownlee M: Oxidative stress and diabetic complications. Circ Res 2010, 107(9):1058-1070.
• [7]Golbidi S, Ebadi SA, Laher I: Antioxidants in the treatment of diabetes. Current Diabetes Rev 2011, 7(2):106-125.
• [8]Resmi CR, Venukumar MR, Latha MS: Antioxidant activity of Albizzia lebbeck (Linn.) Benth. in alloxan diabetic rats. Indian J Physiol Pharmacol 2006, 50(3):297-302.
• [9]Hamden K, Allouche N, Damak M, Elfeki A: Hypoglycemic and antioxidant effects of phenolic extracts and purified hydroxytyrosol from olive mill waste in vitro and in rats. Chemico-Biol Interact 2009, 180(3):421-432.
• [10]Ahmadvand H, Tavafi M, Khalatbary AR: Hepatoprotective and hypolipidemic effects of Satureja Khozestanica essential oil in alloxan-induced Type 1 diabetic rats. Iran J Pharmaceut Res 2012, 11(4):1219-1226.
• [11]Abdollahi M, Salehnia A, Mortazavi SH, Ebrahimi M, Shafiee A, Fouladian F, Keshavarz K, Sorouri S, Khorasani R, Kazemi A: Antioxidant, antidiabetic, antihyperlipidemic, reproduction stimulatory properties and safety of essential oil of Satureja Khuzestanica in rat in vivo: a oxicopharmacological study. Med Sci Monit 2003, 9(9):331-335.
• [12]Ahmadvand H, Tavafi M, Khosrowbeygi A: Amelioration of altered antioxidant enzymes activity and glomerulosclerosis by coenzyme Q10 in alloxan-induced diabetic rats. J Diabetes Complications 2012, 26(6):476-482.
• [13]Buege JA, Aust SD: Microsomal lipid peroxidation. Methods Enz 1957, 52:302-310.
• [14]Ahmadvand H, Bagheri S, Khosrowbeigi A, Boshtam M, Abdolahpour F: Effects of olive leaves extract on LDL oxidation induced-CuSO(4) in vitro. Pak J Pharm Sci 2012, 25(3):571-575.
• [15]Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1979, 72(1/2):248-254.
• [16]Rajasekaran S, Sivagnanam K, Subramanian S: Modulatory effects of Aloe vera leaf gel extract on oxidative stress in rats treated with streptozotocin. J Pharm Pharmacol 2005, 57(2):241-246.
• [17]McMahon M, Skaggs BJ, Sahakian L, Grossman J, Fitz Gerald J, Ragavendra N, Charles-Schoeman C, Chernishof M, Gorn A, Witztum JL, Wong WK, Weisman M, Wallace DJ, La Cava A, Hahn BH: High plasma leptin levels confer increased risk of atherosclerosis in women with systemic lupus erythematosus, and are associated with inflammatory oxidised lipids. Ann Rheum Dis 2011, 70(9):1619-1624.
• [18]Qu ZQ, Zhou Y, Zeng YS, Lin YK, Li Y, Zhong ZQ, Chan WY: Protective Effects of a Rhodiola Crenulata Extract and Salidroside on Hippocampal Neurogenesis against Streptozotocin-Induced Neural Injury in the Rat. PLoS One 2012, 7(1):e29641.
• [19]Wang T, Qiao S, Lei S, Liu Y, Ng KF, Xu A, Lam KS, Irwin MG, Xia Z: N-acetylcysteine and allopurinol synergistically enhance cardiac adiponectin content and reduce myocardial reperfusion injury in diabetic rats. Plos One 2011, 6(8):e23967.
• [20]Moghaddam FM, Farimani MM, Salahvarzi S, Amin G: Chemical con- stituents of dichloromethane extract of cultivated Satureja khuzistanica. Evid Based Complement Alternat Med 2007, 4:95-98.