Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits

BMC Veterinary Research

Yahui Wei¹  Junliang Deng³  Zhihua Ren³  Zhicai Zuo³  Quan Mo³  Yajun He³  Biao Luo³  Yanchun Hu³  Fei Liao²  Yang Hu³ 

[1] Key Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science, Northwest University, Xi’an 710069, China;Qiandongnan Prefectural Center for Animal Disease Control and Prevention of Guizhou province, Kaili 556000, China;Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary, Medicine, Sichuan Agricultural University, Ya an 625014, Sichuan Province, China

关键词: Clinical efficacy;    Acaricidal;    Psoroptes cuniculi;    Eupatorium adenophorum;    Euptox A;   

Others  :  1091554
DOI  :  10.1186/s12917-014-0306-4

 received in 2014-08-08, accepted in 2014-12-13,  发布年份 2014

【 摘 要 】

Background

Animal acariasis is one of the important veterinary skin diseases. Chemical drugs have been widely used to treat and control this kind of disease. But many chemicals control could increase resistance in target species, toxicity and environmental hazards. We found that the 9-oxo-10, 11-dehydroageraphorone (euptox A) extracted from E. adenophorum has strong toxicity against P. cuniculi in vitro, but the in vivo acaricidal actions of euptox A have yet to be investigated.

Results

A 14-day experiment was performed using rabbits that were naturally infested with P. cuniculi on a farm. Rabbits were randomly divided into five groups; animals in groups A, B and C were treated in each ear topically with 4.0 ml of 2.0 and 1.0 g/L (w/v) euptox A, respectively. Animals in groups D and E were treated with ivermectin (by injection; positive controls) and glycerol with water only (by embrocation; negative controls), respectively. Each rabbit was treated twice with separate treatments on days 0 and 7. Rabbits were observed daily and detailed examinations were performed on days 0, 7 and 14, to inspect the presence or absence of mites and scabs/crusts. Seven days after the initial treatment, the mean clinical scores (presence of scabs/crusts) decreased from 3.48, 3.37, 3.43 and 3.45 to 0.37, 0.42, 0.78 and 0.38 in the ears of animals in groups A, B , C and D, respectively, which were similar to the observations recorded in the positive control rabbits. However, the clinical score for negative control rabbits did not increase significantly (P > 0.05) during the experiment, and this changed from 3.32 to 3.37 in the ears, and there were no significant differences in clinical efficacy between left and right ears. After two treatments (0 and 7 d), the rabbits in groups A, B, C and D had recovered completely 14 days after the last treatment and no recurrences of infection were observed.

Conclusions

These results indicate that euptox A was potent compounds for the effective control of animal P. cuniculi in vivo.

【 授权许可】

   
2014 Hu et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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