The interplay between Campylobacter and Helicobacter species and other gastrointestinal microbiota of commercial broiler chickens

Gut Pathogens

Hazel M Mitchell²  Stephen M Riordan³  Julian M Cox¹  Jeremy W Chenu⁴  Nidhi Sodhi²  Nadeem O Kaakoush² 

[1] Faculty of Science, The University of New South Wales, Sydney, NSW 2052, Australia;School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia;Prince of Wales Clinical School, The University of New South Wales, Sydney, NSW 2052, Australia;Birling Avian Laboratories, Bringelly, NSW 2556, Australia

关键词: Campylobacter concisus;    Gallibacterium;    Helicobacter;    Campylobacter;    Pathogen;    Microbiota;    Gastrointestinal tract;    Broiler chicken;   

Others  :  817442
DOI  :  10.1186/1757-4749-6-18

 received in 2014-05-07, accepted in 2014-05-30,  发布年份 2014

【 摘 要 】

Background

Poultry represent an important source of foodborne enteropathogens, in particular thermophilic Campylobacter species. Many of these organisms colonize the intestinal tract of broiler chickens as harmless commensals, and therefore, often remain undetected prior to slaughter. The exact reasons for the lack of clinical disease are unknown, but analysis of the gastrointestinal microbiota of broiler chickens may improve our understanding of the microbial interactions with the host.

Methods

In this study, the fecal microbiota of 31 market-age (56-day old) broiler chickens, from two different farms, was analyzed using high throughput sequencing. The samples were then screened for two emerging human pathogens, Campylobacter concisus and Helicobacter pullorum, using species-specific PCR.

Results

The gastrointestinal microbiota of chickens was classified into four potential enterotypes, similar to that of humans, where three enterotypes have been identified. The results indicated that variations between farms may have contributed to differences in the microbiota, though each of the four enterotypes were found in both farms suggesting that these groupings did not occur by chance. In addition to the identification of Campylobacter jejuni subspecies doylei and the emerging species, C. concisus, C. upsaliensis and H. pullorum, several differences in the prevalence of human pathogens within these enterotypes were observed. Further analysis revealed microbial taxa with the potential to increase the likelihood of colonization by a number of these pathogens, including C. jejuni.

Conclusion

Depletion of these taxa and the addition of taxa that compete with these pathogens, may form the basis of competitive exclusion strategies to eliminate them from the gastrointestinal tract of chickens.

【 授权许可】

   
2014 Kaakoush et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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