Molecular evolution of the leptin exon 3 in some species of the family Canidae

Genetics Selection Evolution

Marek Switonski²  Magdalena Zajac¹  Agata Chmurzynska² 

[1] Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland;Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, Poland

关键词: polymorphism;    Canidae;    leptin;   

Others  :  1094401
DOI  :  10.1186/1297-9686-35-6-573

 received in 2002-09-09, accepted in 2003-02-05,  发布年份 2003

【 摘 要 】

The structure of the leptin gene seems to be well conserved. The polymorphism of this gene in four species belonging to the Canidae family (the dog (Canis familiaris) – 16 different breeds, the Chinese racoon dog (Nyctereutes procyonoides procyonoides), the red fox (Vulpes vulpes) and the arctic fox (Alopex lagopus)) were studied with the use of single strand conformation polymorphism (SSCP), restriction fragment length polymorphism (RFLP) and DNA sequencing techniques. For exon 2, all species presented the same SSCP pattern, while in exon 3 some differences were found. DNA sequencing of exon 3 revealed the presence of six nucleotide substitutions, differentiating the studied species. Three of them cause amino acid substitutions as well. For all dog breeds studied, SSCP patterns were identical.

【 授权许可】

   
2003 INRA, EDP Sciences