Effect of a DIVA vaccine with and without in-feed use of coated calcium-butyrate on transmission of Salmonella Typhimurium in pigs

BMC Veterinary Research

Yves Van der Stede²  Patrick Butaye¹  Bregje Leyman¹  Stefan Roels³  Estelle Méroc⁴  Freddy Haesebrouck¹  Filip Boyen¹  Frank Pasmans¹  Jeroen Dewulf⁵  Dominiek Maes⁵  Lotte De Ridder⁵ 

[1] Department of Pathology, Bacteriology and Avian diseases, Faculty of Veterinary Medicine, Ghent university, Salisburylaan 133, 9820 Merelbeke, Belgium;Department of Virology, parasitology and immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium;Unit of Surveillance, Orientation and Veterinary Support, CODA-CERVA, Groeselenberg 99, 1180 Ukkel, Belgium;Unit of Co-ordination in Veterinary Diagnostics-Epidemiology and Risk Analysis, CODA-CERVA, Groeselenberg 99, 1180 Ukkel, Belgium;Department of Obstetrics, Reproduction and Herd health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium

关键词: Transmission;    Coated calcium-butyrate salt;    DIVA vaccine;    Pig;    Salmonella Typhimurium;   

Others  :  1119395
DOI  :  10.1186/1746-6148-9-243

 received in 2013-07-25, accepted in 2013-11-27,  发布年份 2013

【 摘 要 】

Background

For satisfactory Salmonella control, good biosecurity along the pork production chain is crucial, although additional control measures on-farm need to be considered. This study evaluated the effect of two potential control measures against the spread of Salmonella Typhimurium via a transmission experiment with 56 piglets (3–15 weeks of age): two groups were orally vaccinated with 10⁷ - 10⁸ Colony Forming Units (CFU)/2 mL of a new attenuated Salmonella Typhimurium vaccine ‘Salmoporc-∆rfaJ’ with DIVA capacities (Differentiation between Infected and Vaccinated Animals) (n = 2x16); the feed of one group was additionally supplemented with coated calcium-butyrate salt. Two weeks post vaccination, four pigs per group were orally challenged with 10⁷ CFU/2 mL of a Salmonella Typhimurium strain 112910a. Both groups were compared with a positive (challenged/untreated; n = 16) and negative (unchallenged/untreated; n = 8) control group. Until six weeks post challenge, blood, individual faecal and finally tissue samples were examined. Adjusted transmission ratios ‘R_a’ were estimated, based on the challenge strain isolation from faecal and/or tissue samples.

Results

In both intervention groups, R_a values were lower compared to the positive control group, although these differences were not significant. In the combination group DIVA vaccine + coated butyrate, less non-challenged contact animals excreted Salmonella and less tissue samples were found Salmonella-positive in all pigs, when compared to the positive control group (P < 0.01). Seroconversion was detected in none of the vaccinated animals before challenge, when using a commercial lipopolysaccharide (LPS) ELISA targeting only Salmonella O-antigens, deleted in this vaccine. This was in contrast with an in-house whole-cell ELISA testing for various Salmonella antigens, in which Salmonella-specific antibodies were found pre-challenge in the serum of the vaccinated pigs.

Conclusions

Both interventions showed a limited, non-significant reduction of Salmonella transmission between piglets. They may have applications towards Salmonella control and surveillance. Firstly, the number of Salmonella excreting contact pigs was significantly lower in the group where vaccination was combined with coated calcium-butyrate salt in the feed; secondly, the new vaccine confirmed its DIVA capacity. Therefore, these interventions merit further research with larger sample sizes, to optimize their use for Salmonella programmes.

【 授权许可】

   
2013 De Ridder et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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