Dynamic gene network reconstruction from gene expression data in mice after influenza A (H1N1) infection

Journal of Clinical Bioinformatics

Anastasios Bezerianos⁴  Klaus Schughart¹  Kyriakos N Sgarbas³  Esther Wilk²  Claudia Pommerenke²  George Papadopoulos³  Charalampos Tsimpouris³  Konstantina Dimitrakopoulou⁴ 

[1] University of Veterinary Medicine Hannover, Buenteweg 2, D-30559 Hannover, Germany;Department of Infection Genetics, Helmholtz Centre for Infection Research, Inhoffenstr. 7, D-38124 Braunschweig, Germany;Department of Electrical and Computer Engineering, University of Patras, Patras 26500, Greece;School of Medicine, University of Patras, Patras 26500, Greece

关键词: Influenza A;    Immune System;    Time Varying Dynamic Bayesian Network;    Gene Regulatory Network;   

Others  :  806398
DOI  :  10.1186/2043-9113-1-27

 received in 2011-07-19, accepted in 2011-10-21,  发布年份 2011

【 摘 要 】

Background

The immune response to viral infection is a temporal process, represented by a dynamic and complex network of gene and protein interactions. Here, we present a reverse engineering strategy aimed at capturing the temporal evolution of the underlying Gene Regulatory Networks (GRN). The proposed approach will be an enabling step towards comprehending the dynamic behavior of gene regulation circuitry and mapping the network structure transitions in response to pathogen stimuli.

Results

We applied the Time Varying Dynamic Bayesian Network (TV-DBN) method for reconstructing the gene regulatory interactions based on time series gene expression data for the mouse C57BL/6J inbred strain after infection with influenza A H1N1 (PR8) virus. Initially, 3500 differentially expressed genes were clustered with the use of k-means algorithm. Next, the successive in time GRNs were built over the expression profiles of cluster centroids. Finally, the identified GRNs were examined with several topological metrics and available protein-protein and protein-DNA interaction data, transcription factor and KEGG pathway data.

Conclusions

Our results elucidate the potential of TV-DBN approach in providing valuable insights into the temporal rewiring of the lung transcriptome in response to H1N1 virus.

【 授权许可】

   
2011 Dimitrakopoulou et al; licensee BioMed Central Ltd.

【 图 表 】

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