Restricted nutrient intake does not alter serum-mediated measures of implant response in cell culture

Journal of Animal Science and Biotechnology

Barry J Bradford²  Laman K Mamedova²  Daniel U Thomson¹  Tiffany L Lee¹  Christopher D Reinhardt² 

[1] Department of Clinical Sciences, A-111 Mosier Hall, Kansas State University, Manhattan, KS 66506, USA;Department of Animal Sciences and Industry, 232 Weber Hall, Kansas State University, Manhattan, KS 66506, USA

关键词: Satellite cells;    Nutrient restriction;    Myosin heavy chain;    Muscle;    Implant;    Beef cattle;   

Others  :  804948
DOI  :  10.1186/2049-1891-4-45

 received in 2013-05-31, accepted in 2013-10-22,  发布年份 2013

【 摘 要 】

Background

During nutritional stress, reduced intake may reduce the efficacy of anabolic implants. This study was conducted to evaluate basic cellular responses to a growth promotant implant at two intake levels.

Methods

Sixteen crossbred steers (293 ± 19.3 kg) were used to evaluate the impact of anabolic implants in either an adequate or a restricted nutritional state. Steers were trained to individual Calan gates, and then randomly assigned to 1 of 4 treatments in a 2 × 2 factorial arrangement. Treatments consisted of: presence or absence of an anabolic growth implant (Revalor-XS, 200 mg TBA and 40 mg estradiol; IMPLANT or CONTROL) and a moderate energy, pelleted, starting cattle diet fed at either 2.0 × or 1.0 × maintenance energy (NE_M) requirements (HIGH or LOW). Serum (d 0, 14, and 28) was used for application to bovine muscle satellite cells. After treatment with the serum (20% of total media) from the trial cattle, the satellite cells were incubated for 72 h. Protein abundance of myosin heavy chain (MHC), phosphorylated extracellular signal-related kinase (phospho-ERK), and phosphorylated mammalian target of rapamycin (phospho-mTOR) were analyzed to determine the effects of implant, intake, and their interaction (applied via the serum).

Results

Intake had no effect on MHC (P = 0.85) but IMPLANT increased (P < 0.01) MHC abundance vs. CONTROL. Implant status, intake status, and the interaction had no effect on the abundance of phospho-ERK (P ≥ 0.23). Implanting increased phospho-mTOR (P < 0.01) but there was no effect (P ≥ 0.51) of intake or intake × implant.

Conclusions

The nearly complete lack of interaction between implant and nutritional status indicates that the signaling molecules measured herein respond to implants and nutritional status independently. Furthermore, results suggest that the muscle hypertrophic effects of anabolic implants may not be mediated by circulating IGF-1.

【 授权许可】

   
2013 Reinhardt et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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