Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils

Journal of Environmental Health Science Engineering

Mehrangiz Akbari²  Abdolkarim Chehregani Rad¹  Fariba Mohsenzadeh³ 

[1] Laboratory of Plant Cell Biology, Department of Biology, Bu-Ali Sina University, Hamedan, Iran;Department of Biology, Islamic Azad University, Broujerd Branch, Broujerd, Iran;Laboratory of Microbiology, Department of Biology, Bu-Ali Sina University, Hamedan, Iran

关键词: Soil pollution;    Petroleum removing;    Fungi;    Enzymatic activity;   

Others  :  821431
DOI  :  10.1186/1735-2746-9-26

 received in 2012-07-24, accepted in 2012-12-08,  发布年份 2012

【 摘 要 】

Background

Petroleum pollution is a global disaster and there are several soil cleaning methods including bioremediation.

Methods

In a field study, fugal strains were isolated from oil-contaminated sites of Arak refinery (Iran) and their growth ability was checked in potato dextrose agar (PDA) media containing 0-10% v/v crude oil, the activity of three enzymes (Catalase, Peroxidase and Phenol Oxidase) was evaluated in the fungal colonies and bioremediation ability of the fungi was checked in the experimental pots containing 3 kg sterilized soil and different concentrations of petroleum (0-10% w/w).

Results

Four fungal strains, Acromonium sp., Alternaria sp., Aspergillus terreus and Penicillium sp., were selected as the most resistant ones. They were able to growth in the subjected concentrations and Alternaria sp. showed the highest growth ability in the petroleum containing media. The enzyme assay showed that the enzymatic activity was increased in the oil-contaminated media. Bioremediation results showed that the studied fungi were able to decrease petroleum pollution. The highest petroleum removing efficiency of Aspergillus terreus, Penicillium sp., Alternaria sp. and Acromonium sp. was evaluated in the 10%, 8%, 8% and 2% petroleum pollution respectively.

Conclusions

Fungi are important microorganisms in decreasing of petroleum pollution. They have bioremediation potency that is related to their enzymatic activities.

【 授权许可】

   
2012 Mohsenzadeh et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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