Proton-Induced X-ray Emission (PIXE) Analysis and DNA-chain Break study in rat hepatocarcinogenesis: A possible chemopreventive role by combined supplementation of vanadium and beta-carotene

Cancer Cell International

Malay Chatterjee²  NB Kanjilal²  Manika Doloi³  V Vijayan¹  Indira Kulkarni²  Sutapa Mukherjee²  Mitali Basu Chattopadhyay² 

[1] Institute of Physics, Sachivalaya Marg, Bhubaneswar – 751 005, Orissa, India;Division of Biochemistry, Department of Pharmaceutical Technology Jadavpur University, Kolkata 700 032, India;All Indian Institute of Hygiene and Public Health, Kolkata 700 072, India

关键词: DNA-chain break;    beta-carotene;    PIXE;    vanadium;    DENA;   

Others  :  796001
DOI  :  10.1186/1475-2867-5-16

 received in 2003-08-07, accepted in 2005-05-26,  发布年份 2005

【 摘 要 】

Combined effect of vanadium and beta-carotene on rat liver DNA-chain break and Proton induced X-ray emission (PIXE) analysis was studied during a necrogenic dose (200 mg/kg of body weight) of Diethyl Nitrosamine (DENA) induced rat liver carcinogenesis. Morphological and histopathological changes were observed as an end point biomarker. Supplementation of vanadium (0.5 ppm ad libitum) in drinking water and beta-carotene in the basal diet (120 mg/Kg of body weight) were performed four weeks before DENA treatment and continued till the end of the experiment (16 weeks). PIXE analysis revealed the restoration of near normal value of zinc, copper, and iron, which were substantially altered when compared to carcinogen treated groups. Supplementation of both vanadium and beta-carotene four weeks before DENA injection was found to offer significant (64.73%, P < 0.001) protection against generation of single-strand breaks when compared with the carcinogen control counter parts. A significant stabilization of hepatic architecture of the cells was observed as compared to carcinogen control in vanadium plus beta-carotene treated group. This study thus suggests that vanadium, a prooxidant but potential therapeutic agent yield safe and effective pharmacological formulation with beta-carotene, an antioxidant, in the inhibition of experimental rat hepatocarcinogenesis.

【 授权许可】

   
2005 Chattopadhyay et al; licensee BioMed Central Ltd.

【 图 表 】

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