Antimicrobial effectiveness of silver nanoparticles co-stabilized by the bioactive copolymer pluronic F68

Journal of Nanobiotechnology

Marcelo Martins Seckler¹  Adalberto Pessoa Jr²  Angela Faustino Jozala²  Carolina Alves dos Santos¹ 

[1] Department of Chemical Engineering of the Polytechnic School, University of São Paulo (USP), São Paulo, Brazil;Department of Biochemical and Pharmaceutical Technology, University of São Paulo (USP), São Paulo, Brazil

关键词: Gram-negative;    Gram-positive;    Minimal inhibitory concentration;    Pluronic™;    Polymers;    Silver nanoparticles;   

Others  :  821181
DOI  :  10.1186/1477-3155-10-43

 received in 2012-09-19, accepted in 2012-11-18,  发布年份 2012

【 摘 要 】

Background

Silver nanoparticles (AgNps) have attracted much interest in biomedical engineering, since they have excellent antimicrobial properties. Therefore, AgNps have often been considered for incorporation into medical products for skin pathologies to reduce the risk of contamination. This study aims at evaluating the antimicrobial effectiveness of AgNps stabilized by pluronic™ F68 associated with other polymers such as polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP).

Methods

AgNps antimicrobial activity was evaluated using the minimum inhibitory concentration (MIC) method. The action spectrum was evaluated for different polymers associated with pluronic™ F68 against the gram negative bacteria P. aeuroginosa and E. coli and the gram positive bacteria S. Aureus.

Results

AgNps stabilized with PVP or PVA and co-stabilized with pluronic™ F68 are effective against E. coli and P. aeruginosa microorganisms, with MIC values as low as 0.78% of the concentration of the original AgNps dispersion. The antimicrobial action against S. aureus is poor, with MIC values not lower than 25%.

Conclusions

AgNps stabilized by different polymeric systems have shown improved antimicrobial activity against gram-negative microorganisms in comparison to unstabilized AgNps. Co-stabilization with the bioactive copolymer pluronic™ F68 has further enhanced the antimicrobial effectiveness against both microorganisms. A poor effectiveness has been found against the gram-positive S. aureus microorganism. Future assays are being delineated targeting possible therapeutic applications.

【 授权许可】

   
2012 dos Santos et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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