Journal of Experimental & Clinical Cancer Research | |
miR-212 promotes pancreatic cancer cell growth and invasion by targeting the hedgehog signaling pathway receptor patched-1 | |
Kaixing Ai1  Zhou Yuan1  Xinyu Huang1  Weiwei Wang1  Hongda Zhu1  Yueqing Bai2  Bo Dong1  Bo Wu1  Kate Nong1  Chenchao Ma1  | |
[1] Department of General Surgery, The Sixth People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China;Department of Pathology, The Sixth People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China | |
关键词: Invasion; Migration; Proliferation; Pancreatic cancer; Patched-1; miR-212; | |
Others : 824009 DOI : 10.1186/1756-9966-33-54 |
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received in 2014-04-16, accepted in 2014-06-20, 发布年份 2014 | |
【 摘 要 】
Background
microRNAs (miRNAs) are a class of small non-coding RNAs that play important roles in carcinogenesis. In the present study, we investigated the effect of miR-212 on pancreatic ductal adenocarcinoma (PDAC) and its target protein.
Methods
Quantitative real-time PCR(qRT-PCR) was performed to detect the expression of miR-212 in PDAC tissues and pancreatic cancer cell lines. miR-212 mimic, miR-212 inhibitor and negative control were transfected into pancreatic cancer cells and the effect of miR-212 up-regulation and down-regulation on the proliferation, migration and invasion of cells were investigated. Furthermore, the mRNA and protein levels of Patched-1(PTCH1) were measured. Meanwhile, luciferase assays were performed to validate PTCH1 as miR-212 target in PDAC.
Results
miR-212 was up-regulated in PDAC tissues and cells.Using both gain-of function and loss-of function experiments, a pro-oncogenic function of miR-212 was demonstrated in PDAC. Moreover, up-regulated of PTCH1 could attenuate the effect induced by miR-212.
Conclusion
These data suggest that miR-212 could facilitate PDAC progression and metastasis through targeting PTCH1, implicating a novel mechanism for the progression of PDAC.
【 授权许可】
2014 Ma et al.; licensee BioMed Central Ltd.
【 预 览 】
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20140713021112952.pdf | 2159KB | download | |
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Figure 2. | 84KB | Image | download |
Figure 1. | 43KB | Image | download |
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