Staphylococcal enterotoxin B suppresses Alix and compromises intestinal epithelial barrier functions

Journal of Biomedical Science

Zhigang Liu³  Ping-Chang Yang²  Jijuan Cao¹  Weiyi He²  Zhenke Zhan²  Lixin Xia²  Haitao Yi²  Hao Yan³ 

[1] Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian, China;Shenzhen Key Laboratory of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China;The first affiliated hospital of Liaoning Medical college, Jinzhou, China

关键词: Antigenicity;    Staphylococcal enterotoxin B;    Barrier function;    Epithelial cell;    Alix;   

Others  :  817748
DOI  :  10.1186/1423-0127-21-29

 received in 2013-10-28, accepted in 2014-04-02,  发布年份 2014

【 摘 要 】

Background

The epithelial barrier dysfunction plays a critical role in the pathogenesis of a broad array of immune diseases. Alix protein is involved in the endolysosome system. This study aims to elucidate the role of Alix in the maintenance of epithelial barrier function.

Results

The results showed that Alix was detected in T84 cells at both mRNA and protein levels. Exposure to Staphylococcal enterotoxin B (SEB) markedly suppressed the expression of Alix in T84 cells, which could be blocked by knocking down the Toll like receptor 2. The exposure to SEB did not affect the TER, but markedly increased the permeability of T84 monolayers to OVA; the OVA passing through T84 monolayers still preserved the antigenicity manifesting inducing antigen specific T cells proliferation.

Conclusions

Alix protein plays a critical role in the maintenance of the barrier function of T84 monolayers.

【 授权许可】

   
2014 Yan et al.; licensee BioMed Central Ltd.

【 图 表 】

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