A high-density linkage map of the RN region in pigs

Genetics Selection Evolution

Leif Andersson²  Patrick Chardon¹  Ernst Kalm⁴  Annie Robic³  Valérie Amarger²  Virginie Rey³  Claire Rogel-Gaillard¹  Norbert Reinsch⁴  Sven Paul⁴  Jin-Tae Jeon²  Denis Milan³  Christian Looft⁴ 

[1] Laboratoire de radiobiologie et d'étude du génome, Institut national de la recherche agronomique/Centre énergie atomique, 78352 Jouy-en-Josas Cedex, France;Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, 751 24 Uppsala, Sweden;Laboratoire de génétique cellulaire, Institut national de la recherche agronomique, 31326 Castanet-Tolosan, France;Institute of Animal Breeding and Husbandry, Christian-Albrechts-University, 24098 Kiel, Germany

关键词: genetic mapping;    RN gene;    meat quality;    pig;   

Others  :  1094798
DOI  :  10.1186/1297-9686-32-3-321

 received in 1999-12-06, accepted in 2000-02-10,  发布年份 2000

【 摘 要 】

The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene.

【 授权许可】

   
2000 INRA, EDP Sciences