Substitution of the α-lactalbumin transcription unit by a CAT cDNA within a BAC clone silenced the locus in transgenic mice without affecting the physically linked Cyclin T1 gene

Genetics Selection Evolution

Vilotte Jean-Luc²  Besnard Nathalie²  Viglietta Céline¹  Maeder Caroline¹  Da Silva Costa José²  Hudrisier Marthe²  Soulier Solange² 

[1] Laboratoire de biologie du développement et de biotechnologie, Département de physiologie animale, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France;Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France

关键词: chromatin domain;    transgenic mice;    intron;    homologous recombination;    bacterial artificial chromosome;   

Others  :  1094453
DOI  :  10.1186/1297-9686-35-2-239

 received in 2001-12-12, accepted in 2002-10-01,  发布年份 2003

【 摘 要 】

We recently reported that a goat bacterial artificial chromosome (BAC) clone conferred site-independent expression in transgenic mice of the two loci present within its insert, the ubiquitously expressed Cyclin T1 and the mammary specific β-lactalbumin (αlac) genes. To assess if this vector could target mammary-restricted expression of cDNA, the CAT ORF was introduced by homologous recombination in Escherichia coli in place of the αlac transcription unit. The insert of this modified BAC was injected into mice and three transgenic lines were derived. None of these lines expressed the CAT gene suggesting that the use of long genomic inserts is not sufficient to support the expression of intron-less transgenes. The physically linked goat Cyclin T1 locus was found to be active in all three lines. This observation reinforced the hypothesis that the two loci are localised in two separate chromatin domains.

【 授权许可】

   
2003 INRA, EDP Sciences