Lung progenitors from lambs can differentiate into specialized alveolar or bronchiolar epithelial cells

BMC Veterinary Research

Caroline Leroux¹  Jean-François Mornex²  Vincent Cottin²  François Guiguen¹  Barbara Gineys¹  Christine Dolmazon¹  Sophie Desloire¹  Alain Abi-Rizk¹  Fabienne Archer¹ 

[1] INRA, UMR754, "Rétrovirus et Pathologie Comparée", UMS 3444 SFR BioSciences Gerland - Lyon Sud, Lyon F-69007, France;Hospices Civils de Lyon, Lyon F-69007, France

关键词: Maintenance;    Differentiation;    Sheep;    CD34;    CCSP;    SP-C;    Bronchioloalveolar;    Progenitor;    Lung;   

Others  :  1119414
DOI  :  10.1186/1746-6148-9-224

 received in 2013-04-24, accepted in 2013-10-28,  发布年份 2013

【 摘 要 】

Background

Airways progenitors may be involved in embryogenesis and lung repair. The characterization of these important populations may enable development of new therapeutics to treat acute or chronic lung disease. In this study, we aimed to establish the presence of bronchioloalveolar progenitors in ovine lungs and to characterize their potential to differentiate into specialized cells.

Results

Lung cells were studied using immunohistochemistry on frozen sections of the lung. Immunocytochemistry and flow cytometry were conducted on ex-vivo derived pulmonary cells. The bronchioloalveolar progenitors were identified by their co-expression of CCSP, SP-C and CD34. A minor population of CD34^pos/SP-C^pos/CCSP^pos cells (0.33% ± 0.31) was present ex vivo in cell suspensions from dissociated lungs. Using CD34 magnetic positive-cell sorting, undifferentiated SP-C^pos/CCSP^pos cells were purified (>80%) and maintained in culture. Using synthetic media and various extracellular matrices, SP-C^pos/CCSP^pos cells differentiated into either club cells (formerly named Clara cells) or alveolar epithelial type-II cells. Furthermore, these ex vivo and in vitro derived bronchioloalveolar progenitors expressed NANOG, OCT4 and BMI1, specifically described in progenitors or stem cells, and during lung development.

Conclusions

We report for the first time in a large animal the existence of bronchioloalveolar progenitors with dual differentiation potential and the expression of specialized genes. These newly described cell population in sheep could be implicated in regeneration of the lung following lesions or in development of diseases such as cancers.

【 授权许可】

   
2013 Archer et al.; licensee BioMed Central Ltd.

【 图 表 】

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