Pleiotropic effects of a single gene on skeletal development and sensory system patterning in sticklebacks

EvoDevo

Catherine L Peichel²  Anna K Greenwood²  Margaret G Mills¹ 

[1] Graduate Program in Molecular and Cellular Biology, University of Washington, 1959 NE Pacific Street, Health Sciences Building T-466, Seattle 98195, WA, USA;Divisions of Human Biology and Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle WA, 98109, USA

关键词: Dermal skeleton;    Neuromast;    Lateral line;    Genetics of adaptation;    Pleiotropy;   

Others  :  802225
DOI  :  10.1186/2041-9139-5-5

 received in 2013-10-08, accepted in 2013-11-20,  发布年份 2014

【 摘 要 】

Background

Adaptation to a new environment can be facilitated by co-inheritance of a suite of phenotypes that are all advantageous in the new habitat. Although experimental evidence demonstrates that multiple phenotypes often map to the same genomic regions, it is challenging to determine whether phenotypes are associated due to pleiotropic effects of a single gene or to multiple tightly linked genes. In the threespine stickleback fish (Gasterosteus aculeatus), multiple phenotypes are associated with a genomic region around the gene Ectodysplasin (Eda), but only the presence of bony lateral plates has been conclusively shown to be caused by Eda.

Results

Here, we ask whether pleiotropy or linkage is responsible for the association between lateral plates and the distribution of the neuromasts of the lateral line. We first identify a strong correlation between plate appearance and changes in the spatial distribution of neuromasts through development. We then use an Eda transgene to induce the formation of ectopic plates in low plated fish, which also results in alterations to neuromast distribution. Our results also show that other loci may modify the effects of Eda on plate formation and neuromast distribution.

Conclusions

Together, these results demonstrate that Eda has pleiotropic effects on at least two phenotypes, highlighting the role of pleiotropy in the genetic basis of adaptation.

【 授权许可】

   
2014 Mills et al.; licensee BioMed Central Ltd.

【 图 表 】

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