Immunoreactivities of androgen receptor, estrogen receptors, p450arom, p450c17 proteins in wild ground squirrels ovaries during the nonbreeding and breeding seasons

Journal of Ovarian Research

Kazuyoshi Taya²  Gen Watanabe²  Qiang Weng¹  Meiyu Xu³  Jiao Zhou³  Ben Li³  Xia Sheng³  Haolin Zhang³  Xiaonan Li³ 

[1] Laboratory of Animal Physiology, College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, People’s Republic of China;Department of Basic Science, United Graduate School of Veterinary Sciences, Gifu University, Gifu, 501-1193, Japan;College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China

关键词: Wild ground squirrels;    P450arom;    P450c17;    Ovary;    Androgen receptor;   

Others  :  814973
DOI  :  10.1186/1757-2215-5-26

 received in 2012-09-03, accepted in 2012-09-03,  发布年份 2012

【 摘 要 】

The aim of this study was to elucidate the regulatory role of androgen in the follicular development of wild female ground squirrels. Immunohistochemical staining of FSHR, LHR, P450c17, P450arom, androgen receptor (AR), estrogen receptors (ERa and ERb) were executed in ovaries of female ground squirrels from both breeding and nonbreeding seasons. In addition, total ovarian proteins were extracted from the ovaries of squirrels from breeding and nonbreeding seasons, and Western blot analysis were performed in order to probe for FSHR, LHR, P450c17, P450arom, AR, ERa and ERb. The results of immunohistochemical staining and Western blotting of P450c17 showed that there was no significant difference between the breeding and nonbreeding seasons. It was found that granulosa cells expressed P450arom during the breeding season. In contrast, there was no positive staining of P450arom in the nonbreeding season. There was no significant difference in immunoreactivity of AR between the breeding and nonbreeding seasons. However, the immunoreactivities of ERa and ERb were both significantly reduced in the nonbreeding season compared to the breeding season. The positive stains of FSHR and LHR were found in the granulosa cells and theca cells of the ovaries of the breeding and nonbreeding seasons. In addition, the Western blotting results of FSHR and LHR showed a significant reduction in the nonbreeding season compared with the breeding season. These findings suggested that androgen might be predominantly converted into estrogen in order to regulate the follicular development via binding of estrogen receptors during the breeding season, whereas androgen might predominantly directly bind androgen receptor to regulate the follicular development during the nonbreeding season in the ovaries of wild female ground squirrels.

【 授权许可】

   
2012 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

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