Test for positional candidate genes for body composition on pig chromosome 6

Genetics Selection Evolution

Luis Silió⁴  Miguel Pérez-Enciso¹  Armand Sánchez²  Miguel Toro⁴  Carmen Rodríguez⁴  Luis Varona¹  Carmen Barragán⁴  Alex Clop²  José Luis Noguera¹  Angels Oliver³  Óvilo Cristina⁴ 

[1] Area de Producció Animal, Centre UdL-IRTA, 25198 Lleida, Spain;Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain;Centre de Tecnologia de la Carn, IRTA, 17121 Monells, Girona, Spain;Departamento de Mejora Genética Animal, SGIT-INIA, 28040 Madrid, Spain

关键词: pigs;    QTL;    LEPR;    H-FABP;    candidate gene;   

Others  :  1094536
DOI  :  10.1186/1297-9686-34-4-465

 received in 2001-08-09, accepted in 2002-02-14,  发布年份 2002

【 摘 要 】

One QTL affecting backfat thickness (BF), intramuscular fat content (IMF) and eye muscle area (MA) was previously localized on porcine chromosome 6 in an F₂ cross between Iberian and Landrace pigs. This work was done to study the effect of two positional candidate genes on these traits: H-FABP and LEPR genes. The QTL mapping analysis was repeated with a regression method using genotypes for seven microsatellites and two PCR-RFLPs in the H-FABP and LEPR genes. H-FABP and LEPR genes were located at 85.4 and 107 cM respectively, by linkage analysis. The effects of the candidate gene polymorphisms were analyzed in two ways. When an animal model was fitted, both genes showed significant effects on fatness traits, the H-FABP polymorphism showed significant effects on IMF and MA, and the LEPR polymorphism on BF and IMF. But when the candidate gene effect was included in a QTL regression analysis these associations were not observed, suggesting that they must not be the causal mutations responsible for the effects found. Differences in the results of both analyses showed the inadequacy of the animal model approach for the evaluation of positional candidate genes in populations with linkage disequilibrium, when the probabilities of the parental origin of the QTL alleles are not included in the model.

【 授权许可】

   
2002 INRA, EDP Sciences