Effect of microfracture and autologous-conditioned plasma application in the focal full-thickness chondral defect of the knee: an experimental study on rabbits

Journal of Orthopaedic Surgery and Research

Candan Elmalı³  Emre Ergen¹  Nurhan Şahin⁵  Efe Mirel⁴  Nurzat Elmalı²  Mustafa Karakaplan¹ 

[1] Orthopaedics and Traumatology Department, Turgut Ozal Medical Center, Inonu University Medical School, Malatya 44100, Turkey;Bezmialem Vakıf University, Orthopaedics and Traumatology Clinic, Istanbul, Turkey;Süreyya Pasa Hospital Pathology Clinic, Istanbul, Turkey;Kelkit State Hospital, Orthopaedics and Traumatology Department, Gumushane, Turkey;Pathology Department, Turgut Ozal Medical Center, Inonu University Medical School, Inonu, Malatya, Turkey

关键词: Periosteum;    Platelet-rich plasma;    Cartilage;    Microfracture;   

Others  :  1220951
DOI  :  10.1186/s13018-015-0254-0

 received in 2015-04-28, accepted in 2015-07-01,  发布年份 2015

【 摘 要 】

Purpose

The aim of the present study was to evaluate the effect of microfracture and intraarticular autologous conditioned plasma (ACP) injection on cartilage regeneration in a focal full-thickness chondral defect model created in the knee joint.

Methods

Full-thickness chondral defects of 3 × 6 mm ²were surgically created in right medial femoral condyles (MFC) of New Zealand rabbits, and the rabbits were then divided into three groups according to treatment: Group 1 received only microfracture (mfx), Group 2 received mfx plus intraarticular ACP, and Group 3 received mfx; the defect was covered by the periosteum, and then, ACP was applied subperiosteally and intraarticularly. Twelve weeks after injection, the animals were sacrificed and the femoral condyles were evaluated macroscopically and histologically by hematoxylin-eosin staining. Then, histological sections were scored using the International Cartilage Repair Society (ICRS) visual histological scale.

Results

Findings showed that in both mfx/ACP-treated groups, the defects were filled regularly and smoothly, the defects had a greater fill and good integration into the surrounding host tissue, and the repair matrix had more hyaline-like character. On the other hand, defects were filled with an irregular, fibrous cartilage in the mfx-treated group. Histological scores in Group 2 and Group 3 were better compared to Group 1.

Conclusion

In the present study, we were able to demonstrate a beneficial effect of intraarticular administration of ACP as a coadjuvant of microfractures in order to regenerate hyaline-like cartilage in full-thickness chondral lesions in a rabbit model.

【 授权许可】

   
2015 Karakaplan et al.

【 图 表 】

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