Seasonal changes in expression of nerve growth factor and its receptors TrkA and p75 in the ovary of wild ground squirrel (Citellus dauricus Brandt)

Journal of Ovarian Research

Qiang Weng³  Kazuyoshi Taya²  Gen Watanabe²  Yingying Han³  Yuning Liu³  Qinglin Li³  Shiyang Huang³  Lihong Bao¹  Xia Sheng³  Ben Li³ 

[1] Institute of Public Health, Inner Mongolia University for Nationalities, Tongliao 028000, China;Department of Veterinary Medicine, Faculty of Agriculture, Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan;Laboratory of Animal Physiology, College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China

关键词: TrkA;    p75;    Ovary;    NGF;    Ground squirrel;   

Others  :  802228
DOI  :  10.1186/1757-2215-7-3

 received in 2013-09-20, accepted in 2013-12-21,  发布年份 2014

【 摘 要 】

The aim of this study was to investigate the presence of nerve growth factor (NGF) and its receptors tyrosine kinase A (TrkA) and p75 in the ovaries of the wild ground squirrels during the breeding and nonbreeding seasons. In the breeding period, NGF, TrkA and p75 were immunolocalized in granulosa cells, thecal cells, interstitial cells and luteal cells whereas in the nonbreeding period, both of them were detected only in granulosa cells, thecal cells and interstitial cells. Stronger immunostaining of NGF, TrkA and p75 were observed in granulosa cells, thecal cells and interstitial cells in the breeding season compared to the nonbreeding season. Corresponding for the immunohistochemical results, immunoreactivities of NGF and its two receptors were greater in the ovaries of the breeding season then decreased to a relatively low level in the nonbreeding season. The mean mRNA levels of NGF, TrkA and p75 were significantly higher in the breeding season than in the nonbreeding season. In addition, plasma gonadotropins, estradiol-17β and progesterone concentrations were significantly higher in the breeding season than in the nonbreeding season, suggesting that the expression patterns of NGF, and TrkA and p75 were correlated with changes in plasma gonadotropins, estradiol-17β and progesterone concentrations. These results indicated that NGF and its receptors, TrkA and p75 may be involved in the regulation of seasonal changes in the ovarian functions of the wild ground squirrel.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

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