lobChIP: from cells to sequencing ready ChIP libraries in a single day

Epigenetics & Chromatin

Claes Wadelius³  Lisa Borghini⁴  Madhusudhan Bysani²  Helena Nord³  Ola Wallerman¹ 

[1] Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden;Department of Clinical Sciences, CRC, Lund University Diabetes Center, Malmö, Sweden;Science for Life Laboratory, Department of Immunology, Genetics and Pathology, BMC, Uppsala University, Uppsala, 75108, Sweden;Human Genetics, Genome Institute of Singapore, Singapore, Singapore

关键词: Library preparation;    NGS;    Illumina;    Chromatin immunoprecipitation;    ChIP-seq;   

Others  :  1221287
DOI  :  10.1186/s13072-015-0017-5

 received in 2015-06-22, accepted in 2015-07-08,  发布年份 2015

【 摘 要 】

Background

ChIP-seq is the method of choice for genome-wide studies of protein–DNA interactions. We describe a new method for ChIP-seq sample preparation, termed lobChIP, where the library reactions are performed on cross-linked ChIP fragments captured on beads.

Results

The lobChIP method was found both to reduce time and cost and to simplify the processing of many samples in parallel. lobChIP has an early incorporation of barcoded sequencing adaptors that minimizes the risk of sample cross-contamination and can lead to reduced amount of adaptor dimers in the sequencing libraries, while allowing for direct decross-linking and amplification of the sample.

Conclusions

With results for histone modifications and transcription factors, we show that lobChIP performs equal to or better than standard protocols and that it makes it possible to go from cells to sequencing ready libraries within a single day.

【 授权许可】

   
2015 Wallerman et al.

【 图 表 】

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