Impact of Meyerozyma guilliermondii isolated from chickens against Eimeria sp. protozoan, an in vitro analysis

BMC Veterinary Research

Laura Ortiz-Hernández⁴  Leobardo Serrano-Carreón¹  José Raunel Tinoco-Valencia¹  Yitzel Gama-Martínez²  Jorge Valle-Hernández⁴  Mayra Cobaxin-Cárdenas⁴  Rosa Estela Quiroz-Castañeda³  Edgar Dantán-González⁴ 

[1] Unidad de Escalamiento y Planta Piloto-Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico;Facltad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico;Unidad de Anaplasmosis del CENID-Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Juitepec, Morelos, Mexico;Laboratorio de Investigaciones Ambientales-Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico

关键词: Anticoccidial;    Meyerozyma guilliermondii;    Oocyst;    Avian coccidiosis;   

Others  :  1234001
DOI  :  10.1186/s12917-015-0589-0

 received in 2015-04-07, accepted in 2015-10-30,  发布年份 2015

【 摘 要 】

Background

Avian coccidiosis is a disease caused worldwide by several species of parasite Eimeria that causes significant economic losses. This disease affects chickens development and production, that most of times is controlled with anticoccidial drugs. Although efforts have been made to address this disease, they have been made to control Eimeria sporozoites, although enteric stages are often vulnerable, however; the parasite oocyst remains a problem that must be controlled, as it has a resistant structure that facilitates dispersion. Despite some commercial products based on chemical compounds have been developed as disinfectants that destroy oocysts, the solution of the problem remains to be solved.

Results

In this work, we assessed in vitro anticoccidial activity of a compound(s) secreted by yeast isolated in oocysts suspension from infected chickens. The yeast was molecularly identified as Meyerozyma guilliermondii, and its anticoccidial activity against Eimeria tenella oocysts was assessed. Here, we report the damage to oocysts walls caused by M. guilliermondii culture, supernatant, supernatant extract and intracellular proteins. In all cases, a significant decreased of oocysts was observed.

Conclusions

The yeast Meyerozyma guilliermondii secretes a compound with anticoccidial activity and also has a compound of protein nature that damages the resistant structure of oocyst, showing the potential of this yeast and its products as a feasible method of coccidiosis control.

【 授权许可】

   
2015 Dantán-González et al.

【 图 表 】

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig 5.

【 参考文献 】

• [1]Wiedmer S, Stange J, Kurth T, Bleiss W, Entzeroth R, Kurth M. New insights into the excystation process and oocyst morphology of rodent Eimeria species. Protist. 2011; 162:668-678.
• [2]Lal K, Bromley E, Oakes R, Prieto JH, Sanderson SJ, Kurian D, Hunt L, Yates JR, Wastling JM, Sinden RE et al.. Proteomic comparison of four Eimeria tenella life cycle stages: unsporulated oocyst, sporulated oocyst, sporozoite and second generation merozoite. Proteomics. 2009; 9(19):4566-4576.
• [3]McDonald V, Shirley MW. Past and future: vaccination against Eimeria. Parasitology. 2009; 136:1477-1489.
• [4]Blake D, Tomley FM. Securing poultry production from the ever-present Eimeria challenge. Trends Parasitol. 2014; 30(1):12-19.
• [5]Dalloul RA, Lillehoj HS. Recent advances in immunomodulation and vaccination strategies against coccidiosis. Avian Dis. 2005; 49:1-8.
• [6]Gilbert ER, Cox CM, Williams PM, McElroy AP, Dalloul RA, Ray WK, Barri A, Emmerson DA, Wong EA, Webb KE. Eimeria species and genetic background influence the serum protein profile of broilers with coccidiosis. PLoS One. 2011; 6(1):e14636.
• [7]Haug A, Gjevre AG, Thebo P, Mattsson JG, Kaldhusdal M. Coccidial infections in commercial broilers: epidemiological aspects and comparison of Eimeria species identification by morphometric and polymerase chain reaction techniques. Avian Pathol. 2008; 37:161-170.
• [8]McDougald LR. Protozoal Infections. In: Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LR, Swayne DE, editors. Diseases of poultry. Ames, IA, USA: Iowa State University Press; 2003. pp. 973–1023.
• [9]Norton CC, Chard MJ. The oocyst sporulation time of Eimeria species from the fowl. Parasitology. 1983; 86:193-198.
• [10]Belli SI, Smith NC, Ferguson DJ. The coccidian oocyst: a tough nut to crack! Trends Parasitol. 2006; 22:416-423.
• [11]Mai K, Sharman PA, Walker RA, Katrib M, De Souza D, McConville MJ, Wallach MG, Belli SI, Ferguson DJ, Smith NC. Oocyst wall formation and composition in coccidian parasites. Mem Inst Oswaldo Cruz. 2009; 104(2):281-289.
• [12]Schares G, Pantchev N, Barutzki D, Heydorn AO, Bauer C, Conraths FJ. Oocysts of Neospora caninum, Hammondia heydorni, Toxoplasma gondii and Hammondia hammondi in faeces collected from dogs in Germany. Int J Parasitol. 2005; 35:1525-1537.
• [13]Chapman HD, Jeffers TK, Williams RB. Forty years of monensin for the control of coccidiosis in poultry. Poultry Sci. 2010; 89:1788-1801.
• [14]Abbas RZ, Iqbal Z, Blake D, Khan MN, Saleemi MK. Anticoccidial drug resistance in fowl coccidia: the state of play revisited. World’s Poul Sci J. 2011; 67(2):337-350.
• [15]Remmal A, Achahbar S, Bouddine L, Chami F, Chami N. Oocysticidal effect of essential oil components against chicken Eimeria oocysts. Int J Vet Med. 2013; 2013:599816.
• [16]Remmal A, Achahbar S, Bouddine L, Chami N, Chami F. In vitro destruction of Eimeria oocysts by essential oils. Vet Parasitol. 2011; 182:121-126.
• [17]Abbas RZ, Colwell DD, Gilleard J. Botanicals: an alternative approach for the control of avian coccidiosis. World’s Poul Sci J. 2012; 68(2):203-215.
• [18]Bozkurt M, Selek N, Küçükyilmaz K, Eren H, Güven E, Çatli AU, Çinar M. Effects of dietary supplementation with a herbal extract on the performance of broilers infected with a mixture of Eimeria species. Brit Poultry Sci. 2012; 53(3):325-332.
• [19]Quiroz-Castañeda RE, Dantán-González E. Control of avian coccidiosis: future and present natural alternatives. BioMed Res Int. 2015; 2015:430610.
• [20]Villa-Carvajal M, Querol A, Belloch C. Identification of species in the genus Pichia by restriction of the internal transcribed spacers (ITS1 and ITS2) and the 5.8S ribosomal DNA gene. Antonie Van Leeuwenhoek. 2006; 90(2):171-181.
• [21]White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications. Innis MA, Gelfand DH, Shinsky JJ, White TJ, editors. Academic, San Diego; 1990: p.315-322.
• [22]Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek. 1998; 73(4):331-371.
• [23]Tamura S, Stecher G, Peterson D, Filipski A, Kumar S. MEGA 6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol Biol Evol. 2013; 30:2725-2729.
• [24]Bradford MM. A dye binding assay for protein. Anal Biochem. 1976; 72:248-254.
• [25]Kurtzman C, Suzuki M. Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera: Babjeviella, Meyerozyma, Millerozyma, Priceomyces and Scheffersomyces. Mycoscience. 2010; 51:2-14.
• [26]Prasad GS. Sequence anlysis of internal transcribed spacer region for phylogenetics assessment of ascomycetous yeast. Microbial diversity current perspectives and potential applications. Satyanarayana T, Johri BN, editors. I.K. International Publishing House Pvt. Ltd, New Delhi; 2005.
• [27]Arroyo-López FN, Durán-Quintana MC, Ruiz-Barba JL, Querol A, Garrido-Fernández A. Use of molecular methods for the identification of yeast associated with table olives. Food Microbiol. 2006; 23:791-796.
• [28]Wickerham LJ. Validation of the species Pichia guilliermondii. J Bacteriol. 1966; 92(4):1269.
• [29]Shanmugasundaram R, Sifri M, Selvaraj RM. Effect of yeast cell product (CitriStim) supplementation on broiler performance and intestinal immune cell parameters during an experimental coccidial infection. Poultry Sci. 2013; 92:358-363.
• [30]Zhao J, Mou Y, Shan T, Li Y, Zhou L, Wang M, Wang J. Antimicrobial metabolites from the endophytic fungus Pichia guilliermondii isolated from Paris polyphylla var. yunnanensis. Molecules. 2010; 15:7961-7970.